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Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action
OBJECTIVE: This study was performed to confirm the anti-inflammatory effect of the Mongolian drug Naru-3 on traumatic spinal cord injury (TSCI) and its possible mechanism of action. METHODS: We prepared a TSCI model using Sprague–Dawley rats. The rats were divided into a Naru-3 group and a methylpre...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023071/ https://www.ncbi.nlm.nih.gov/pubmed/29614905 http://dx.doi.org/10.1177/0300060518760157 |
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author | Baiyila, Bulin He, Bing He, Guisong Long, Tengfei |
author_facet | Baiyila, Bulin He, Bing He, Guisong Long, Tengfei |
author_sort | Baiyila, Bulin |
collection | PubMed |
description | OBJECTIVE: This study was performed to confirm the anti-inflammatory effect of the Mongolian drug Naru-3 on traumatic spinal cord injury (TSCI) and its possible mechanism of action. METHODS: We prepared a TSCI model using Sprague–Dawley rats. The rats were divided into a Naru-3 group and a methylprednisolone group. Real-time polymerase chain reaction and western blotting were performed to measure the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Enzyme-linked immunosorbent assay kits were employed to detect serum inflammatory cytokine levels. The localization and expression of monocyte chemotactic protein-1 (MCP-1) in spinal cord tissue was determined by immunohistochemical analysis. Flow cytometry was performed to analyze the ratio of M1- and M2-phenotype macrophages. The locomotor function recovery was evaluated by the Basso, Beattie, and Bresnahan score. RESULTS: Naru-3 significantly inhibited the inflammatory response and reduced the expression of TNF-α, IL-6, and IL-1β in both spinal cord and blood in a time- and concentration-dependent manner. Immunohistochemical analysis indicated that Naru-3 significantly reduced MCP-1 expression in spinal cord and promoted M2-phenotype macrophage differentiation. CONCLUSIONS: Naru-3 is an effective treatment for impact-induced TSCI in rats. Naru-3 treatment affects inflammatory cytokine levels and macrophage differentiation, which play a role in TSCI remission. |
format | Online Article Text |
id | pubmed-6023071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-60230712018-07-05 Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action Baiyila, Bulin He, Bing He, Guisong Long, Tengfei J Int Med Res Pre-Clinical Research Reports OBJECTIVE: This study was performed to confirm the anti-inflammatory effect of the Mongolian drug Naru-3 on traumatic spinal cord injury (TSCI) and its possible mechanism of action. METHODS: We prepared a TSCI model using Sprague–Dawley rats. The rats were divided into a Naru-3 group and a methylprednisolone group. Real-time polymerase chain reaction and western blotting were performed to measure the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Enzyme-linked immunosorbent assay kits were employed to detect serum inflammatory cytokine levels. The localization and expression of monocyte chemotactic protein-1 (MCP-1) in spinal cord tissue was determined by immunohistochemical analysis. Flow cytometry was performed to analyze the ratio of M1- and M2-phenotype macrophages. The locomotor function recovery was evaluated by the Basso, Beattie, and Bresnahan score. RESULTS: Naru-3 significantly inhibited the inflammatory response and reduced the expression of TNF-α, IL-6, and IL-1β in both spinal cord and blood in a time- and concentration-dependent manner. Immunohistochemical analysis indicated that Naru-3 significantly reduced MCP-1 expression in spinal cord and promoted M2-phenotype macrophage differentiation. CONCLUSIONS: Naru-3 is an effective treatment for impact-induced TSCI in rats. Naru-3 treatment affects inflammatory cytokine levels and macrophage differentiation, which play a role in TSCI remission. SAGE Publications 2018-04-03 2018-06 /pmc/articles/PMC6023071/ /pubmed/29614905 http://dx.doi.org/10.1177/0300060518760157 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Reports Baiyila, Bulin He, Bing He, Guisong Long, Tengfei Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title | Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title_full | Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title_fullStr | Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title_full_unstemmed | Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title_short | Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action |
title_sort | anti-inflammatory effect of mongolian drug naru-3 on traumatic spinal cord injury and its mechanism of action |
topic | Pre-Clinical Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023071/ https://www.ncbi.nlm.nih.gov/pubmed/29614905 http://dx.doi.org/10.1177/0300060518760157 |
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