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Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae

The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to anti...

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Autores principales: Rørbo, Nanna, Rønneseth, Anita, Kalatzis, Panos G., Rasmussen, Bastian Barker, Engell-Sørensen, Kirsten, Kleppen, Hans Petter, Wergeland, Heidrun Inger, Gram, Lone, Middelboe, Mathias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023099/
https://www.ncbi.nlm.nih.gov/pubmed/29772736
http://dx.doi.org/10.3390/antibiotics7020042
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author Rørbo, Nanna
Rønneseth, Anita
Kalatzis, Panos G.
Rasmussen, Bastian Barker
Engell-Sørensen, Kirsten
Kleppen, Hans Petter
Wergeland, Heidrun Inger
Gram, Lone
Middelboe, Mathias
author_facet Rørbo, Nanna
Rønneseth, Anita
Kalatzis, Panos G.
Rasmussen, Bastian Barker
Engell-Sørensen, Kirsten
Kleppen, Hans Petter
Wergeland, Heidrun Inger
Gram, Lone
Middelboe, Mathias
author_sort Rørbo, Nanna
collection PubMed
description The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V. anguillarum strains in Atlantic cod (Gadus morhua L.) and turbot (Scophthalmus maximus L.) larvae. We examined larval mortality and abundance of bacteria and phages. Phage KVP40 was able to reduce and/or delay the mortality of the cod and turbot larvae challenged with V. anguillarum. However, growth of other pathogenic bacteria naturally occurring on the fish eggs prior to our experiment caused mortality of the larvae in the unchallenged control groups. Interestingly, the broad-spectrum phage KVP40 was able to reduce mortality in these groups, compared to the nonchallenge control groups not treated with phage KVP40, demonstrating that the phage could also reduce mortality imposed by the background population of pathogens. Overall, phage-mediated reduction in mortality of cod and turbot larvae in experimental challenge assays with V. anguillarum pathogens suggested that application of broad-host-range phages can reduce Vibrio-induced mortality in turbot and cod larvae, emphasizing that phage therapy is a promising alternative to traditional treatment of vibriosis in marine aquaculture.
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spelling pubmed-60230992018-07-02 Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae Rørbo, Nanna Rønneseth, Anita Kalatzis, Panos G. Rasmussen, Bastian Barker Engell-Sørensen, Kirsten Kleppen, Hans Petter Wergeland, Heidrun Inger Gram, Lone Middelboe, Mathias Antibiotics (Basel) Article The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V. anguillarum strains in Atlantic cod (Gadus morhua L.) and turbot (Scophthalmus maximus L.) larvae. We examined larval mortality and abundance of bacteria and phages. Phage KVP40 was able to reduce and/or delay the mortality of the cod and turbot larvae challenged with V. anguillarum. However, growth of other pathogenic bacteria naturally occurring on the fish eggs prior to our experiment caused mortality of the larvae in the unchallenged control groups. Interestingly, the broad-spectrum phage KVP40 was able to reduce mortality in these groups, compared to the nonchallenge control groups not treated with phage KVP40, demonstrating that the phage could also reduce mortality imposed by the background population of pathogens. Overall, phage-mediated reduction in mortality of cod and turbot larvae in experimental challenge assays with V. anguillarum pathogens suggested that application of broad-host-range phages can reduce Vibrio-induced mortality in turbot and cod larvae, emphasizing that phage therapy is a promising alternative to traditional treatment of vibriosis in marine aquaculture. MDPI 2018-05-16 /pmc/articles/PMC6023099/ /pubmed/29772736 http://dx.doi.org/10.3390/antibiotics7020042 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rørbo, Nanna
Rønneseth, Anita
Kalatzis, Panos G.
Rasmussen, Bastian Barker
Engell-Sørensen, Kirsten
Kleppen, Hans Petter
Wergeland, Heidrun Inger
Gram, Lone
Middelboe, Mathias
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title_full Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title_fullStr Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title_full_unstemmed Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title_short Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
title_sort exploring the effect of phage therapy in preventing vibrio anguillarum infections in cod and turbot larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023099/
https://www.ncbi.nlm.nih.gov/pubmed/29772736
http://dx.doi.org/10.3390/antibiotics7020042
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