Cargando…

Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali

Neural stem cells (NSCs) are important cellular sources of transplantation therapies for Parkinson’s disease. This study aimed to determine the effects of extracts of radix astragali on the proliferation and differentiation into dopamine (DA) neurons in NSCs. NSCs were dealt with astragaloside IV (A...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Han, Dou, Lianrong, Shan, Liang, Sun, Yan, Li, Wentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023595/
https://www.ncbi.nlm.nih.gov/pubmed/29481521
http://dx.doi.org/10.1097/WNR.0000000000000997
_version_ 1783335899369570304
author Gao, Han
Dou, Lianrong
Shan, Liang
Sun, Yan
Li, Wentao
author_facet Gao, Han
Dou, Lianrong
Shan, Liang
Sun, Yan
Li, Wentao
author_sort Gao, Han
collection PubMed
description Neural stem cells (NSCs) are important cellular sources of transplantation therapies for Parkinson’s disease. This study aimed to determine the effects of extracts of radix astragali on the proliferation and differentiation into dopamine (DA) neurons in NSCs. NSCs were dealt with astragaloside IV (ASI), astragalus polysaccharide (APS), and astraisoflavan (ASF), the main active ingredients of radix astragali. First, the results from cell-count kit-8 (CCK-8) assay showed that ASI, ASF, and APS had positive effects on the proliferation of NSCs. Next, we also confirmed the effects of ASI, APS, and ASF on BrdU and nestin by immunocytochemistry. Moreover, results from quantitative RT-PCR showed ASI, APS, and ASF could promote the expressions of tyrosine hydroxylase and dopamine transporter mRNA, which are specifically expressed in DA neurons. Simultaneously, sonic hedgehog (Shh), orphan nuclear hormone 1 (Nurr1), and pituitary homeobox 3 (Ptx3) are considered to motivate the formation of DA neurons. Our result showed ASI, APS, and ASF can also promote the expressions of Shh, Nurr1, and Ptx3 mRNAs. In conclusion, our study verifies that the active ingredients of radix astragali can promote the proliferation of NSCs and induce NSC differentiation toward DA neurons in vitro. These phenomena may occur through upregulation of Shh, Nurr1, and Ptx3 in the process of drug treatment.
format Online
Article
Text
id pubmed-6023595
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-60235952018-07-11 Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali Gao, Han Dou, Lianrong Shan, Liang Sun, Yan Li, Wentao Neuroreport Degeneration and Repair Neural stem cells (NSCs) are important cellular sources of transplantation therapies for Parkinson’s disease. This study aimed to determine the effects of extracts of radix astragali on the proliferation and differentiation into dopamine (DA) neurons in NSCs. NSCs were dealt with astragaloside IV (ASI), astragalus polysaccharide (APS), and astraisoflavan (ASF), the main active ingredients of radix astragali. First, the results from cell-count kit-8 (CCK-8) assay showed that ASI, ASF, and APS had positive effects on the proliferation of NSCs. Next, we also confirmed the effects of ASI, APS, and ASF on BrdU and nestin by immunocytochemistry. Moreover, results from quantitative RT-PCR showed ASI, APS, and ASF could promote the expressions of tyrosine hydroxylase and dopamine transporter mRNA, which are specifically expressed in DA neurons. Simultaneously, sonic hedgehog (Shh), orphan nuclear hormone 1 (Nurr1), and pituitary homeobox 3 (Ptx3) are considered to motivate the formation of DA neurons. Our result showed ASI, APS, and ASF can also promote the expressions of Shh, Nurr1, and Ptx3 mRNAs. In conclusion, our study verifies that the active ingredients of radix astragali can promote the proliferation of NSCs and induce NSC differentiation toward DA neurons in vitro. These phenomena may occur through upregulation of Shh, Nurr1, and Ptx3 in the process of drug treatment. Lippincott Williams & Wilkins 2018-05-02 2018-02-26 /pmc/articles/PMC6023595/ /pubmed/29481521 http://dx.doi.org/10.1097/WNR.0000000000000997 Text en Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Degeneration and Repair
Gao, Han
Dou, Lianrong
Shan, Liang
Sun, Yan
Li, Wentao
Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title_full Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title_fullStr Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title_full_unstemmed Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title_short Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
title_sort proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali
topic Degeneration and Repair
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023595/
https://www.ncbi.nlm.nih.gov/pubmed/29481521
http://dx.doi.org/10.1097/WNR.0000000000000997
work_keys_str_mv AT gaohan proliferationandcommitteddifferentiationintodopamineneuronsofneuralstemcellsinducedbytheactiveingredientsofradixastragali
AT doulianrong proliferationandcommitteddifferentiationintodopamineneuronsofneuralstemcellsinducedbytheactiveingredientsofradixastragali
AT shanliang proliferationandcommitteddifferentiationintodopamineneuronsofneuralstemcellsinducedbytheactiveingredientsofradixastragali
AT sunyan proliferationandcommitteddifferentiationintodopamineneuronsofneuralstemcellsinducedbytheactiveingredientsofradixastragali
AT liwentao proliferationandcommitteddifferentiationintodopamineneuronsofneuralstemcellsinducedbytheactiveingredientsofradixastragali