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TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-depende...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023610/ https://www.ncbi.nlm.nih.gov/pubmed/29911973 http://dx.doi.org/10.7554/eLife.36144 |
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author | Ladam, Franck Stanney, William Donaldson, Ian J Yildiz, Ozge Bobola, Nicoletta Sagerström, Charles G |
author_facet | Ladam, Franck Stanney, William Donaldson, Ian J Yildiz, Ozge Bobola, Nicoletta Sagerström, Charles G |
author_sort | Ladam, Franck |
collection | PubMed |
description | TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages – a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs. |
format | Online Article Text |
id | pubmed-6023610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60236102018-07-05 TALE factors use two distinct functional modes to control an essential zebrafish gene expression program Ladam, Franck Stanney, William Donaldson, Ian J Yildiz, Ozge Bobola, Nicoletta Sagerström, Charles G eLife Developmental Biology TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages – a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs. eLife Sciences Publications, Ltd 2018-06-18 /pmc/articles/PMC6023610/ /pubmed/29911973 http://dx.doi.org/10.7554/eLife.36144 Text en © 2018, Ladam et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Ladam, Franck Stanney, William Donaldson, Ian J Yildiz, Ozge Bobola, Nicoletta Sagerström, Charles G TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title | TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title_full | TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title_fullStr | TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title_full_unstemmed | TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title_short | TALE factors use two distinct functional modes to control an essential zebrafish gene expression program |
title_sort | tale factors use two distinct functional modes to control an essential zebrafish gene expression program |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023610/ https://www.ncbi.nlm.nih.gov/pubmed/29911973 http://dx.doi.org/10.7554/eLife.36144 |
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