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TALE factors use two distinct functional modes to control an essential zebrafish gene expression program

TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-depende...

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Autores principales: Ladam, Franck, Stanney, William, Donaldson, Ian J, Yildiz, Ozge, Bobola, Nicoletta, Sagerström, Charles G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023610/
https://www.ncbi.nlm.nih.gov/pubmed/29911973
http://dx.doi.org/10.7554/eLife.36144
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author Ladam, Franck
Stanney, William
Donaldson, Ian J
Yildiz, Ozge
Bobola, Nicoletta
Sagerström, Charles G
author_facet Ladam, Franck
Stanney, William
Donaldson, Ian J
Yildiz, Ozge
Bobola, Nicoletta
Sagerström, Charles G
author_sort Ladam, Franck
collection PubMed
description TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages – a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs.
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spelling pubmed-60236102018-07-05 TALE factors use two distinct functional modes to control an essential zebrafish gene expression program Ladam, Franck Stanney, William Donaldson, Ian J Yildiz, Ozge Bobola, Nicoletta Sagerström, Charles G eLife Developmental Biology TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages – a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs. eLife Sciences Publications, Ltd 2018-06-18 /pmc/articles/PMC6023610/ /pubmed/29911973 http://dx.doi.org/10.7554/eLife.36144 Text en © 2018, Ladam et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Ladam, Franck
Stanney, William
Donaldson, Ian J
Yildiz, Ozge
Bobola, Nicoletta
Sagerström, Charles G
TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title_full TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title_fullStr TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title_full_unstemmed TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title_short TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
title_sort tale factors use two distinct functional modes to control an essential zebrafish gene expression program
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023610/
https://www.ncbi.nlm.nih.gov/pubmed/29911973
http://dx.doi.org/10.7554/eLife.36144
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