Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic modifications with single base-pair precision. The preference of mammalian cells to repair Cas9-induced DNA double-strand breaks via error-prone end-joining pathways rather than via homology-directed repair mechanisms, howe...

Descripción completa

Detalles Bibliográficos
Autores principales: Savic, Natasa, Ringnalda, Femke CAS, Lindsay, Helen, Berk, Christian, Bargsten, Katja, Li, Yizhou, Neri, Dario, Robinson, Mark D, Ciaudo, Constance, Hall, Jonathan, Jinek, Martin, Schwank, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Chromosomes and Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023611/
https://www.ncbi.nlm.nih.gov/pubmed/29809142
http://dx.doi.org/10.7554/eLife.33761

Ejemplares similares

Ejemplares similares