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SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension
The study aimed to characterize the expression and function of SFTA3 at the ocular surface and in tears. Ocular tissues, conjunctival (HCjE) and human corneal (HCE) epithelial cell lines as well as tearfilm of patients suffering from different forms of dry eye disease (DED) were analyzed by means of...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023927/ https://www.ncbi.nlm.nih.gov/pubmed/29955092 http://dx.doi.org/10.1038/s41598-018-28005-9 |
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author | Schicht, Martin Garreis, Fabian Hartjen, Nadine Beileke, Stephanie Jacobi, Christina Sahin, Afsun Holland, Detlef Schröder, Henrik Hammer, Christian M. Paulsen, Friedrich Bräuer, Lars |
author_facet | Schicht, Martin Garreis, Fabian Hartjen, Nadine Beileke, Stephanie Jacobi, Christina Sahin, Afsun Holland, Detlef Schröder, Henrik Hammer, Christian M. Paulsen, Friedrich Bräuer, Lars |
author_sort | Schicht, Martin |
collection | PubMed |
description | The study aimed to characterize the expression and function of SFTA3 at the ocular surface and in tears. Ocular tissues, conjunctival (HCjE) and human corneal (HCE) epithelial cell lines as well as tearfilm of patients suffering from different forms of dry eye disease (DED) were analyzed by means of RT-PCR, western blot, immunohistochemistry, and ELISA. A possible role of recombinant SFTA3 in corneal wound healing was investigated performing in vitro scratch assays. Tear film regulatory properties were analyzed with the spinning drop method and the regulation of SFTA3 transcripts was studied in HCE and HCjE after incubation with proinflammatory cytokines as well as typical ocular pathogens by real-time RT-PCR and ELISA. The results reveal that human ocular tissue as well as tears of healthy volunteers express SFTA3 whereas tears from patients with DED showed significantly increased SFTA3 levels. In vitro wounding of HCE cell cultures that had been treated with recombinant SFTA3 demonstrated a significantly increased wound closure rate and rSFTA3 reduced the surface tension of tear fluid. The results indicate that SFTA3 at the ocular surface seemed to be involved in wound healing and the reduction of surface tension. |
format | Online Article Text |
id | pubmed-6023927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60239272018-07-06 SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension Schicht, Martin Garreis, Fabian Hartjen, Nadine Beileke, Stephanie Jacobi, Christina Sahin, Afsun Holland, Detlef Schröder, Henrik Hammer, Christian M. Paulsen, Friedrich Bräuer, Lars Sci Rep Article The study aimed to characterize the expression and function of SFTA3 at the ocular surface and in tears. Ocular tissues, conjunctival (HCjE) and human corneal (HCE) epithelial cell lines as well as tearfilm of patients suffering from different forms of dry eye disease (DED) were analyzed by means of RT-PCR, western blot, immunohistochemistry, and ELISA. A possible role of recombinant SFTA3 in corneal wound healing was investigated performing in vitro scratch assays. Tear film regulatory properties were analyzed with the spinning drop method and the regulation of SFTA3 transcripts was studied in HCE and HCjE after incubation with proinflammatory cytokines as well as typical ocular pathogens by real-time RT-PCR and ELISA. The results reveal that human ocular tissue as well as tears of healthy volunteers express SFTA3 whereas tears from patients with DED showed significantly increased SFTA3 levels. In vitro wounding of HCE cell cultures that had been treated with recombinant SFTA3 demonstrated a significantly increased wound closure rate and rSFTA3 reduced the surface tension of tear fluid. The results indicate that SFTA3 at the ocular surface seemed to be involved in wound healing and the reduction of surface tension. Nature Publishing Group UK 2018-06-28 /pmc/articles/PMC6023927/ /pubmed/29955092 http://dx.doi.org/10.1038/s41598-018-28005-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schicht, Martin Garreis, Fabian Hartjen, Nadine Beileke, Stephanie Jacobi, Christina Sahin, Afsun Holland, Detlef Schröder, Henrik Hammer, Christian M. Paulsen, Friedrich Bräuer, Lars SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title | SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title_full | SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title_fullStr | SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title_full_unstemmed | SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title_short | SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
title_sort | sfta3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023927/ https://www.ncbi.nlm.nih.gov/pubmed/29955092 http://dx.doi.org/10.1038/s41598-018-28005-9 |
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