Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)

Our previous study showed that the inactivation of the efflux pump TolC could abolish biofilm formation and curli production of extraintestinal pathogenic Escherichia coli (ExPEC) strain PPECC42 under hyper-osmotic conditions. In this study we investigated the role of OmpX in biofilm formation and c...

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Autores principales: Li, Binyou, Huang, Qi, Cui, Ailian, Liu, Xueling, Hou, Bo, Zhang, Liyuan, Liu, Mei, Meng, Xianrong, Li, Shaowen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024092/
https://www.ncbi.nlm.nih.gov/pubmed/29988395
http://dx.doi.org/10.3389/fcimb.2018.00208
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author Li, Binyou
Huang, Qi
Cui, Ailian
Liu, Xueling
Hou, Bo
Zhang, Liyuan
Liu, Mei
Meng, Xianrong
Li, Shaowen
author_facet Li, Binyou
Huang, Qi
Cui, Ailian
Liu, Xueling
Hou, Bo
Zhang, Liyuan
Liu, Mei
Meng, Xianrong
Li, Shaowen
author_sort Li, Binyou
collection PubMed
description Our previous study showed that the inactivation of the efflux pump TolC could abolish biofilm formation and curli production of extraintestinal pathogenic Escherichia coli (ExPEC) strain PPECC42 under hyper-osmotic conditions. In this study we investigated the role of OmpX in biofilm formation and curli production of ExPEC PPECC42. Our data showed that OmpX disruption or overexpression didn't significantly affect the biofilm formation and curli production of the wild-type strain. However, in the tolC-deleted mutant, overexpressing OmpX suppressed the effect of TolC inactivation on ExPEC biofilm formation and curli production under hyper-osmotic growth conditions. Real-time qRT-PCR confirmed that OmpX overexpression affected curli production by regulating the transcription of the curli biosynthesis-related genes in the ΔtolC strain. Our findings suggest that OmpX is involved in biofilm formation and curli production.
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spelling pubmed-60240922018-07-09 Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC) Li, Binyou Huang, Qi Cui, Ailian Liu, Xueling Hou, Bo Zhang, Liyuan Liu, Mei Meng, Xianrong Li, Shaowen Front Cell Infect Microbiol Cellular and Infection Microbiology Our previous study showed that the inactivation of the efflux pump TolC could abolish biofilm formation and curli production of extraintestinal pathogenic Escherichia coli (ExPEC) strain PPECC42 under hyper-osmotic conditions. In this study we investigated the role of OmpX in biofilm formation and curli production of ExPEC PPECC42. Our data showed that OmpX disruption or overexpression didn't significantly affect the biofilm formation and curli production of the wild-type strain. However, in the tolC-deleted mutant, overexpressing OmpX suppressed the effect of TolC inactivation on ExPEC biofilm formation and curli production under hyper-osmotic growth conditions. Real-time qRT-PCR confirmed that OmpX overexpression affected curli production by regulating the transcription of the curli biosynthesis-related genes in the ΔtolC strain. Our findings suggest that OmpX is involved in biofilm formation and curli production. Frontiers Media S.A. 2018-06-22 /pmc/articles/PMC6024092/ /pubmed/29988395 http://dx.doi.org/10.3389/fcimb.2018.00208 Text en Copyright © 2018 Li, Huang, Cui, Liu, Hou, Zhang, Liu, Meng and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Binyou
Huang, Qi
Cui, Ailian
Liu, Xueling
Hou, Bo
Zhang, Liyuan
Liu, Mei
Meng, Xianrong
Li, Shaowen
Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title_full Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title_fullStr Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title_full_unstemmed Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title_short Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal Pathogenic Escherichia coli (ExPEC)
title_sort overexpression of outer membrane protein x (ompx) compensates for the effect of tolc inactivation on biofilm formation and curli production in extraintestinal pathogenic escherichia coli (expec)
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024092/
https://www.ncbi.nlm.nih.gov/pubmed/29988395
http://dx.doi.org/10.3389/fcimb.2018.00208
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