Cargando…
Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats
Salvianolic acid A (SAA) is an active phenolic acid derived from Salvia miltiorrhiza Bunge (Danshen). To explore whether SAA has a therapeutic effect against inflammatory bowel disease (IBD), an acute colitis model was induced in rats by administering 3% dextran sodium sulphate (DSS) for one week. S...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024375/ https://www.ncbi.nlm.nih.gov/pubmed/29921812 http://dx.doi.org/10.3390/nu10060791 |
_version_ | 1783336050294259712 |
---|---|
author | Wang, Kai Yang, Qinqin Ma, Quanxin Wang, Bei Wan, Zhengrui Chen, Minli Wu, Liming |
author_facet | Wang, Kai Yang, Qinqin Ma, Quanxin Wang, Bei Wan, Zhengrui Chen, Minli Wu, Liming |
author_sort | Wang, Kai |
collection | PubMed |
description | Salvianolic acid A (SAA) is an active phenolic acid derived from Salvia miltiorrhiza Bunge (Danshen). To explore whether SAA has a therapeutic effect against inflammatory bowel disease (IBD), an acute colitis model was induced in rats by administering 3% dextran sodium sulphate (DSS) for one week. SAA in doses of 4 and 8 mg/kg/day was given by tail vein injection during DSS administration. Both dosages of SAA ameliorated the colitis symptoms, with decreases observed in the disease activity index. A high dosage of SAA (8 mg/kg/day) promoted a longer colon length and an improved colonic tissue structure, compared with the DSS-treated rats not receiving SAA. SAA dose-dependently decreased colonic gene expression of pro-inflammatory cytokines (IL-1β, MCP-1 and IL-6). Moreover, a high dosage of SAA protected against DSS-induced damage to tight junctions (TJ) in the rats’ colons, by increasing TJ-related gene expression (ZO-1 and occuldin). Finally, using 16S rRNA phylogenetic sequencing, we found that SAA modulated gut microbiota imbalance during colitis by increasing the gut microbial diversity as well as selectively promoting some probiotic populations, including Akkermansia spp. Our study suggests that SAA is a promising candidate for the treatment of IBD. |
format | Online Article Text |
id | pubmed-6024375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60243752018-07-08 Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats Wang, Kai Yang, Qinqin Ma, Quanxin Wang, Bei Wan, Zhengrui Chen, Minli Wu, Liming Nutrients Article Salvianolic acid A (SAA) is an active phenolic acid derived from Salvia miltiorrhiza Bunge (Danshen). To explore whether SAA has a therapeutic effect against inflammatory bowel disease (IBD), an acute colitis model was induced in rats by administering 3% dextran sodium sulphate (DSS) for one week. SAA in doses of 4 and 8 mg/kg/day was given by tail vein injection during DSS administration. Both dosages of SAA ameliorated the colitis symptoms, with decreases observed in the disease activity index. A high dosage of SAA (8 mg/kg/day) promoted a longer colon length and an improved colonic tissue structure, compared with the DSS-treated rats not receiving SAA. SAA dose-dependently decreased colonic gene expression of pro-inflammatory cytokines (IL-1β, MCP-1 and IL-6). Moreover, a high dosage of SAA protected against DSS-induced damage to tight junctions (TJ) in the rats’ colons, by increasing TJ-related gene expression (ZO-1 and occuldin). Finally, using 16S rRNA phylogenetic sequencing, we found that SAA modulated gut microbiota imbalance during colitis by increasing the gut microbial diversity as well as selectively promoting some probiotic populations, including Akkermansia spp. Our study suggests that SAA is a promising candidate for the treatment of IBD. MDPI 2018-06-19 /pmc/articles/PMC6024375/ /pubmed/29921812 http://dx.doi.org/10.3390/nu10060791 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Kai Yang, Qinqin Ma, Quanxin Wang, Bei Wan, Zhengrui Chen, Minli Wu, Liming Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title | Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title_full | Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title_fullStr | Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title_full_unstemmed | Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title_short | Protective Effects of Salvianolic Acid A against Dextran Sodium Sulfate-Induced Acute Colitis in Rats |
title_sort | protective effects of salvianolic acid a against dextran sodium sulfate-induced acute colitis in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024375/ https://www.ncbi.nlm.nih.gov/pubmed/29921812 http://dx.doi.org/10.3390/nu10060791 |
work_keys_str_mv | AT wangkai protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT yangqinqin protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT maquanxin protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT wangbei protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT wanzhengrui protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT chenminli protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats AT wuliming protectiveeffectsofsalvianolicacidaagainstdextransodiumsulfateinducedacutecolitisinrats |