NeuO for Neuronal Labeling in Zebrafish

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting Neu...

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Detalles Bibliográficos
Autores principales: Teoh, Chai Lean, Er, Jun Cheng, Mukherjee, Parag, Chang, Young-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Grapho Publications, LLC 2015
Materias:
Advances in Brief
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024405/
https://www.ncbi.nlm.nih.gov/pubmed/30042954
http://dx.doi.org/10.18383/j.tom.2015.00127
Descripción
Sumario:We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.

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