NeuO for Neuronal Labeling in Zebrafish

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting Neu...

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Detalles Bibliográficos
Autores principales: Teoh, Chai Lean, Er, Jun Cheng, Mukherjee, Parag, Chang, Young-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Grapho Publications, LLC 2015
Materias:
Advances in Brief
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024405/
https://www.ncbi.nlm.nih.gov/pubmed/30042954
http://dx.doi.org/10.18383/j.tom.2015.00127
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author Teoh, Chai Lean
Er, Jun Cheng
Mukherjee, Parag
Chang, Young-Tae
author_facet Teoh, Chai Lean
Er, Jun Cheng
Mukherjee, Parag
Chang, Young-Tae
author_sort Teoh, Chai Lean
collection PubMed
description We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.
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spelling pubmed-60244052018-07-24 NeuO for Neuronal Labeling in Zebrafish Teoh, Chai Lean Er, Jun Cheng Mukherjee, Parag Chang, Young-Tae Tomography Advances in Brief We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration. Grapho Publications, LLC 2015-09 /pmc/articles/PMC6024405/ /pubmed/30042954 http://dx.doi.org/10.18383/j.tom.2015.00127 Text en © 2015 The Authors. Published by Grapho Publications, LLC http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Advances in Brief
Teoh, Chai Lean
Er, Jun Cheng
Mukherjee, Parag
Chang, Young-Tae
NeuO for Neuronal Labeling in Zebrafish
title NeuO for Neuronal Labeling in Zebrafish
title_full NeuO for Neuronal Labeling in Zebrafish
title_fullStr NeuO for Neuronal Labeling in Zebrafish
title_full_unstemmed NeuO for Neuronal Labeling in Zebrafish
title_short NeuO for Neuronal Labeling in Zebrafish
title_sort neuo for neuronal labeling in zebrafish
topic Advances in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024405/
https://www.ncbi.nlm.nih.gov/pubmed/30042954
http://dx.doi.org/10.18383/j.tom.2015.00127
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AT erjuncheng neuoforneuronallabelinginzebrafish
AT mukherjeeparag neuoforneuronallabelinginzebrafish
AT changyoungtae neuoforneuronallabelinginzebrafish

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