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A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish

Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcrip...

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Autores principales: Liao, Qiwen, Gong, Guiyi, Siu, Shirley Weng In, Wong, Clarence Tsun Ting, Yu, Huidong, Tse, Yu Chung, Rádis-Baptista, Gandhi, Lee, Simon Ming-Yuen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024583/
https://www.ncbi.nlm.nih.gov/pubmed/29895785
http://dx.doi.org/10.3390/toxins10060238
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author Liao, Qiwen
Gong, Guiyi
Siu, Shirley Weng In
Wong, Clarence Tsun Ting
Yu, Huidong
Tse, Yu Chung
Rádis-Baptista, Gandhi
Lee, Simon Ming-Yuen
author_facet Liao, Qiwen
Gong, Guiyi
Siu, Shirley Weng In
Wong, Clarence Tsun Ting
Yu, Huidong
Tse, Yu Chung
Rádis-Baptista, Gandhi
Lee, Simon Ming-Yuen
author_sort Liao, Qiwen
collection PubMed
description Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcriptome of P. caribaeroum was deep sequenced, and a diversity of toxin-related peptide sequences were identified, and some retrieved for functional analysis. In this work, a peptide precursor containing a ShK domain, named PcShK3, was analyzed by means of computational processing, comprising structural phylogenetic analysis, model prediction, and dynamics simulation of peptide-receptor interaction. The combined data indicated that PcShK3 is a distinct peptide which is homologous to a cluster of peptides belonging to the ShK toxin family. In vivo, PcShK3 distributed across the vitelline membrane and accumulated in the yolk sac stripe of zebrafish larvae. Notably, it displayed a significant cardio-protective effect in zebrafish in concentrations inferior to the IC(50) (<43.53 ± 6.45 µM), while in high concentrations (>IC(50)), it accumulated in the blood and caused pericardial edema, being cardiotoxic to zebrafish larvae. Remarkably, PcShK3 suppressed the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish. The present results indicated that PcShK3 is a novel member of ShK toxin family, and has the intrinsic ability to induce neuro- and cardio-protective effects or cause cardiac toxicity, according to its effective concentration.
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spelling pubmed-60245832018-07-09 A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish Liao, Qiwen Gong, Guiyi Siu, Shirley Weng In Wong, Clarence Tsun Ting Yu, Huidong Tse, Yu Chung Rádis-Baptista, Gandhi Lee, Simon Ming-Yuen Toxins (Basel) Article Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcriptome of P. caribaeroum was deep sequenced, and a diversity of toxin-related peptide sequences were identified, and some retrieved for functional analysis. In this work, a peptide precursor containing a ShK domain, named PcShK3, was analyzed by means of computational processing, comprising structural phylogenetic analysis, model prediction, and dynamics simulation of peptide-receptor interaction. The combined data indicated that PcShK3 is a distinct peptide which is homologous to a cluster of peptides belonging to the ShK toxin family. In vivo, PcShK3 distributed across the vitelline membrane and accumulated in the yolk sac stripe of zebrafish larvae. Notably, it displayed a significant cardio-protective effect in zebrafish in concentrations inferior to the IC(50) (<43.53 ± 6.45 µM), while in high concentrations (>IC(50)), it accumulated in the blood and caused pericardial edema, being cardiotoxic to zebrafish larvae. Remarkably, PcShK3 suppressed the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish. The present results indicated that PcShK3 is a novel member of ShK toxin family, and has the intrinsic ability to induce neuro- and cardio-protective effects or cause cardiac toxicity, according to its effective concentration. MDPI 2018-06-12 /pmc/articles/PMC6024583/ /pubmed/29895785 http://dx.doi.org/10.3390/toxins10060238 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liao, Qiwen
Gong, Guiyi
Siu, Shirley Weng In
Wong, Clarence Tsun Ting
Yu, Huidong
Tse, Yu Chung
Rádis-Baptista, Gandhi
Lee, Simon Ming-Yuen
A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title_full A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title_fullStr A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title_full_unstemmed A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title_short A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
title_sort novel shk-like toxic peptide from the transcriptome of the cnidarian palythoa caribaeorum displays neuroprotection and cardioprotection in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024583/
https://www.ncbi.nlm.nih.gov/pubmed/29895785
http://dx.doi.org/10.3390/toxins10060238
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