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Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies

Babesia bigemina is one of the most prevalent species causing bovine babesiosis around the world. Antigens involved in host cell invasion are vaccine targets for this disease but are largely unknown in this species. The invasion process of Babesia spp. into erythrocytes involves membrane proteins fr...

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Autores principales: Hernández-Silva, Diego Josimar, Valdez-Espinoza, Uriel Mauricio, Mercado-Uriostegui, Miguel Angel, Aguilar-Tipacamú, Gabriela, Ramos-Aragón, Juan Alberto, Hernández-Ortiz, Ruben, Ueti, Massaro, Mosqueda, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024600/
https://www.ncbi.nlm.nih.gov/pubmed/29570654
http://dx.doi.org/10.3390/vetsci5020032
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author Hernández-Silva, Diego Josimar
Valdez-Espinoza, Uriel Mauricio
Mercado-Uriostegui, Miguel Angel
Aguilar-Tipacamú, Gabriela
Ramos-Aragón, Juan Alberto
Hernández-Ortiz, Ruben
Ueti, Massaro
Mosqueda, Juan
author_facet Hernández-Silva, Diego Josimar
Valdez-Espinoza, Uriel Mauricio
Mercado-Uriostegui, Miguel Angel
Aguilar-Tipacamú, Gabriela
Ramos-Aragón, Juan Alberto
Hernández-Ortiz, Ruben
Ueti, Massaro
Mosqueda, Juan
author_sort Hernández-Silva, Diego Josimar
collection PubMed
description Babesia bigemina is one of the most prevalent species causing bovine babesiosis around the world. Antigens involved in host cell invasion are vaccine targets for this disease but are largely unknown in this species. The invasion process of Babesia spp. into erythrocytes involves membrane proteins from the apical complex. A protein stored in the micronemes, called Micronemal Protein 1 (MIC-1), contains a sialic acid binding domain that participates in the invasion process of host cells and is a vaccine candidate in other apicomplexan parasites. It is not known if there is a homologous gene for mic-1 in B. bigemina. Therefore, the aim of this study was to characterize the mic-1 gene homologue in Babesia bigemina. A gene was found with a microneme adhesive repeat (MAR) domain in the predicted amino acid sequence. Transcription was determined by reverse transcription polymerase chain reaction (RT-PCR). Subsequently, antibodies against peptides containing conserved B-cell epitopes were used to confirm the expression of MIC-1 in intraerythrocytic merozoites. The presence of anti MIC-1 antibodies in cattle naturally infected with B. bigemina was determined and up to 97.4% of the cattle sera (113 out of 116) identified MIC-1 using enzyme-linked immunosorbent assay (ELISA) methods. Finally, antibodies against MIC-1 were able to block 70% merozoite invasion in-vitro.
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spelling pubmed-60246002018-07-08 Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies Hernández-Silva, Diego Josimar Valdez-Espinoza, Uriel Mauricio Mercado-Uriostegui, Miguel Angel Aguilar-Tipacamú, Gabriela Ramos-Aragón, Juan Alberto Hernández-Ortiz, Ruben Ueti, Massaro Mosqueda, Juan Vet Sci Article Babesia bigemina is one of the most prevalent species causing bovine babesiosis around the world. Antigens involved in host cell invasion are vaccine targets for this disease but are largely unknown in this species. The invasion process of Babesia spp. into erythrocytes involves membrane proteins from the apical complex. A protein stored in the micronemes, called Micronemal Protein 1 (MIC-1), contains a sialic acid binding domain that participates in the invasion process of host cells and is a vaccine candidate in other apicomplexan parasites. It is not known if there is a homologous gene for mic-1 in B. bigemina. Therefore, the aim of this study was to characterize the mic-1 gene homologue in Babesia bigemina. A gene was found with a microneme adhesive repeat (MAR) domain in the predicted amino acid sequence. Transcription was determined by reverse transcription polymerase chain reaction (RT-PCR). Subsequently, antibodies against peptides containing conserved B-cell epitopes were used to confirm the expression of MIC-1 in intraerythrocytic merozoites. The presence of anti MIC-1 antibodies in cattle naturally infected with B. bigemina was determined and up to 97.4% of the cattle sera (113 out of 116) identified MIC-1 using enzyme-linked immunosorbent assay (ELISA) methods. Finally, antibodies against MIC-1 were able to block 70% merozoite invasion in-vitro. MDPI 2018-03-23 /pmc/articles/PMC6024600/ /pubmed/29570654 http://dx.doi.org/10.3390/vetsci5020032 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Silva, Diego Josimar
Valdez-Espinoza, Uriel Mauricio
Mercado-Uriostegui, Miguel Angel
Aguilar-Tipacamú, Gabriela
Ramos-Aragón, Juan Alberto
Hernández-Ortiz, Ruben
Ueti, Massaro
Mosqueda, Juan
Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title_full Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title_fullStr Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title_full_unstemmed Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title_short Immunomolecular Characterization of MIC-1, a Novel Antigen in Babesia bigemina, Which Contains Conserved and Immunodominant B-Cell Epitopes that Induce Neutralizing Antibodies
title_sort immunomolecular characterization of mic-1, a novel antigen in babesia bigemina, which contains conserved and immunodominant b-cell epitopes that induce neutralizing antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024600/
https://www.ncbi.nlm.nih.gov/pubmed/29570654
http://dx.doi.org/10.3390/vetsci5020032
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