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A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers

Vitamin K was originally discovered as a cofactor required to activate clotting factors and has recently been shown to play a key role in the regulation of soft tissue calcification. This property of vitamin K has led to an increased interest in novel methods for accurate vitamin K detection. Molecu...

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Autores principales: Eersels, Kasper, Diliën, Hanne, Lowdon, Joseph W., Steen Redeker, Erik, Rogosic, Renato, Heidt, Benjamin, Peeters, Marloes, Cornelis, Peter, Lux, Petra, Reutelingsperger, Chris P., Schurgers, Leon J., Cleij, Thomas J., van Grinsven, Bart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024727/
https://www.ncbi.nlm.nih.gov/pubmed/29891757
http://dx.doi.org/10.3390/nu10060751
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author Eersels, Kasper
Diliën, Hanne
Lowdon, Joseph W.
Steen Redeker, Erik
Rogosic, Renato
Heidt, Benjamin
Peeters, Marloes
Cornelis, Peter
Lux, Petra
Reutelingsperger, Chris P.
Schurgers, Leon J.
Cleij, Thomas J.
van Grinsven, Bart
author_facet Eersels, Kasper
Diliën, Hanne
Lowdon, Joseph W.
Steen Redeker, Erik
Rogosic, Renato
Heidt, Benjamin
Peeters, Marloes
Cornelis, Peter
Lux, Petra
Reutelingsperger, Chris P.
Schurgers, Leon J.
Cleij, Thomas J.
van Grinsven, Bart
author_sort Eersels, Kasper
collection PubMed
description Vitamin K was originally discovered as a cofactor required to activate clotting factors and has recently been shown to play a key role in the regulation of soft tissue calcification. This property of vitamin K has led to an increased interest in novel methods for accurate vitamin K detection. Molecularly Imprinted Polymers (MIPs) could offer a solution, as they have been used as synthetic receptors in a large variety of biomimetic sensors for the detection of similar molecules over the past few decades, because of their robust nature and remarkable selectivity. In this article, the authors introduce a novel imprinting approach to create a MIP that is able to selectively rebind vitamin K(1). As the native structure of the vitamin does not allow for imprinting, an alternative imprinting strategy was developed, using the synthetic compound menadione (vitamin K(3)) as a template. Target rebinding was analyzed by means of UV-visible (UV-VIS) spectroscopy and two custom-made thermal readout techniques. This analysis reveals that the MIP-based sensor reacts to an increasing concentration of both menadione and vitamin K(1). The Limit of Detection (LoD) for both compounds was established at 700 nM for the Heat Transfer Method (HTM), while the optimized readout approach, Thermal Wave Transport Analysis (TWTA), displayed an increased sensitivity with a LoD of 200 nM. The sensor seems to react to a lesser extent to Vitamin E, the analogue under study. To further demonstrate its potential application in biochemical research, the sensor was used to measure the absorption of vitamin K in blood serum after taking vitamin K supplements. By employing a gradual enrichment strategy, the sensor was able to detect the difference between baseline and peak absorption samples and was able to quantify the vitamin K concentration in good agreement with a validation experiment using High-Performance Liquid Chromatography (HPLC). In this way, the authors provide a first proof of principle for a low-cost, straightforward, and label-free vitamin K sensor.
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spelling pubmed-60247272018-07-08 A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers Eersels, Kasper Diliën, Hanne Lowdon, Joseph W. Steen Redeker, Erik Rogosic, Renato Heidt, Benjamin Peeters, Marloes Cornelis, Peter Lux, Petra Reutelingsperger, Chris P. Schurgers, Leon J. Cleij, Thomas J. van Grinsven, Bart Nutrients Article Vitamin K was originally discovered as a cofactor required to activate clotting factors and has recently been shown to play a key role in the regulation of soft tissue calcification. This property of vitamin K has led to an increased interest in novel methods for accurate vitamin K detection. Molecularly Imprinted Polymers (MIPs) could offer a solution, as they have been used as synthetic receptors in a large variety of biomimetic sensors for the detection of similar molecules over the past few decades, because of their robust nature and remarkable selectivity. In this article, the authors introduce a novel imprinting approach to create a MIP that is able to selectively rebind vitamin K(1). As the native structure of the vitamin does not allow for imprinting, an alternative imprinting strategy was developed, using the synthetic compound menadione (vitamin K(3)) as a template. Target rebinding was analyzed by means of UV-visible (UV-VIS) spectroscopy and two custom-made thermal readout techniques. This analysis reveals that the MIP-based sensor reacts to an increasing concentration of both menadione and vitamin K(1). The Limit of Detection (LoD) for both compounds was established at 700 nM for the Heat Transfer Method (HTM), while the optimized readout approach, Thermal Wave Transport Analysis (TWTA), displayed an increased sensitivity with a LoD of 200 nM. The sensor seems to react to a lesser extent to Vitamin E, the analogue under study. To further demonstrate its potential application in biochemical research, the sensor was used to measure the absorption of vitamin K in blood serum after taking vitamin K supplements. By employing a gradual enrichment strategy, the sensor was able to detect the difference between baseline and peak absorption samples and was able to quantify the vitamin K concentration in good agreement with a validation experiment using High-Performance Liquid Chromatography (HPLC). In this way, the authors provide a first proof of principle for a low-cost, straightforward, and label-free vitamin K sensor. MDPI 2018-06-11 /pmc/articles/PMC6024727/ /pubmed/29891757 http://dx.doi.org/10.3390/nu10060751 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eersels, Kasper
Diliën, Hanne
Lowdon, Joseph W.
Steen Redeker, Erik
Rogosic, Renato
Heidt, Benjamin
Peeters, Marloes
Cornelis, Peter
Lux, Petra
Reutelingsperger, Chris P.
Schurgers, Leon J.
Cleij, Thomas J.
van Grinsven, Bart
A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title_full A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title_fullStr A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title_full_unstemmed A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title_short A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
title_sort novel biomimetic tool for assessing vitamin k status based on molecularly imprinted polymers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024727/
https://www.ncbi.nlm.nih.gov/pubmed/29891757
http://dx.doi.org/10.3390/nu10060751
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