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PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population
Aims: The PR domain containing 16 (PRDM16) gene and the Phosphodiesterase 4D (PDE4) gene are both an essential regulators in the thermogenesis process in the brown adipose tissues (BAT). The influence of polymorphisms in those genes on obesity and blood lipids profile is unknown particularly in the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025268/ https://www.ncbi.nlm.nih.gov/pubmed/29890628 http://dx.doi.org/10.3390/jcm7060141 |
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author | AlAmrani, Aishah AbdelKarim, Mouaadh AlZoghaibi, Mohammed |
author_facet | AlAmrani, Aishah AbdelKarim, Mouaadh AlZoghaibi, Mohammed |
author_sort | AlAmrani, Aishah |
collection | PubMed |
description | Aims: The PR domain containing 16 (PRDM16) gene and the Phosphodiesterase 4D (PDE4) gene are both an essential regulators in the thermogenesis process in the brown adipose tissues (BAT). The influence of polymorphisms in those genes on obesity and blood lipids profile is unknown particularly in the Saudi population, so the current study is aiming to explore that. Methods: A case control format was used that involved 89 obese individual and 84 non-obese (control). The PRDM16 (rs2651899) and PDE4D (rs295978) polymorphisms were genotyped using KASP™ (Competitive Allele-Specific PCR) method. Results: The distributions of the AA, GG, and AG genotypes of PRDM16 (rs2651899) polymorphism were 0.19, 0.26 and 0.54, respectively. While the distribution of the mutated allele A was 0.7 in the obese group comparing to 0.34 in the non-obese group. Participants with the mutated genotypes, AA and AG, of PRDM16 (rs2651899) polymorphism were significantly more likely to be obese as compared to participants with wild type genotype (OR = 21, 95% CI = 5.4190 to 84.4231, p value < 0.0001 and OR = 44.6, 95% CI = 11.5984 to 172.0157, p value < 0.0001, respectively). The wild type GG genotype of this polymorphism was associated with higher blood cholesterol, HDL and LDL but lower blood triglyceride compared with the mutated genotypes (p = 0.003, p = 0.008, p = 0.02 and p = 0.003, respectively). In contrast, PDE4D (rs295978) polymorphism was not associated with risk of obesity and had no effects on blood lipids profile. Conclusions: We found that the PRDM16 polymorphism (rs2651899) is a risk factor for obesity and influence blood lipids profiles significantly in Saudi population. While the PDE4D (rs295978) polymorphism didn’t show significant effect on risk of obesity or blood lipids profiles. |
format | Online Article Text |
id | pubmed-6025268 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60252682018-07-09 PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population AlAmrani, Aishah AbdelKarim, Mouaadh AlZoghaibi, Mohammed J Clin Med Article Aims: The PR domain containing 16 (PRDM16) gene and the Phosphodiesterase 4D (PDE4) gene are both an essential regulators in the thermogenesis process in the brown adipose tissues (BAT). The influence of polymorphisms in those genes on obesity and blood lipids profile is unknown particularly in the Saudi population, so the current study is aiming to explore that. Methods: A case control format was used that involved 89 obese individual and 84 non-obese (control). The PRDM16 (rs2651899) and PDE4D (rs295978) polymorphisms were genotyped using KASP™ (Competitive Allele-Specific PCR) method. Results: The distributions of the AA, GG, and AG genotypes of PRDM16 (rs2651899) polymorphism were 0.19, 0.26 and 0.54, respectively. While the distribution of the mutated allele A was 0.7 in the obese group comparing to 0.34 in the non-obese group. Participants with the mutated genotypes, AA and AG, of PRDM16 (rs2651899) polymorphism were significantly more likely to be obese as compared to participants with wild type genotype (OR = 21, 95% CI = 5.4190 to 84.4231, p value < 0.0001 and OR = 44.6, 95% CI = 11.5984 to 172.0157, p value < 0.0001, respectively). The wild type GG genotype of this polymorphism was associated with higher blood cholesterol, HDL and LDL but lower blood triglyceride compared with the mutated genotypes (p = 0.003, p = 0.008, p = 0.02 and p = 0.003, respectively). In contrast, PDE4D (rs295978) polymorphism was not associated with risk of obesity and had no effects on blood lipids profile. Conclusions: We found that the PRDM16 polymorphism (rs2651899) is a risk factor for obesity and influence blood lipids profiles significantly in Saudi population. While the PDE4D (rs295978) polymorphism didn’t show significant effect on risk of obesity or blood lipids profiles. MDPI 2018-06-08 /pmc/articles/PMC6025268/ /pubmed/29890628 http://dx.doi.org/10.3390/jcm7060141 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article AlAmrani, Aishah AbdelKarim, Mouaadh AlZoghaibi, Mohammed PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title | PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title_full | PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title_fullStr | PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title_full_unstemmed | PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title_short | PRDM16 Gene Polymorphism Is Associated with Obesity and Blood Lipids Profiles in Saudi Population |
title_sort | prdm16 gene polymorphism is associated with obesity and blood lipids profiles in saudi population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025268/ https://www.ncbi.nlm.nih.gov/pubmed/29890628 http://dx.doi.org/10.3390/jcm7060141 |
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