A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis

Fluorescence lifetime imaging (FLIM) is a powerful imaging modality used to gather fluorescent reporter data independent of intracellular reporter intensity or imaging depth. We applied this technique to image real-time activation of a reporter for the proteolytic enzyme, caspase-3, in response to a...

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Detalles Bibliográficos
Autores principales: Buschhaus, Johanna M., Humphries, Brock, Luker, Kathryn E., Luker, Gary D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025355/
https://www.ncbi.nlm.nih.gov/pubmed/30720785
http://dx.doi.org/10.3390/cells7060057

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