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Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans

Physical forces are transduced into chemical reactions, thereby ultimately making a large impact on the whole-animal level phenotypes such as homeostasis, development and behavior. To understand mechano-chemical transduction, mechanical input should be quantitatively delivered with controllable vibr...

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Detalles Bibliográficos
Autores principales: Sugi, Takuma, Igarashi, Ryuji, Nishimura, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025516/
https://www.ncbi.nlm.nih.gov/pubmed/29921777
http://dx.doi.org/10.3390/ma11061034
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author Sugi, Takuma
Igarashi, Ryuji
Nishimura, Masaki
author_facet Sugi, Takuma
Igarashi, Ryuji
Nishimura, Masaki
author_sort Sugi, Takuma
collection PubMed
description Physical forces are transduced into chemical reactions, thereby ultimately making a large impact on the whole-animal level phenotypes such as homeostasis, development and behavior. To understand mechano-chemical transduction, mechanical input should be quantitatively delivered with controllable vibration properties–frequency, amplitude and duration, and its chemical output should be noninvasively quantified in an unconstrained animal. However, such an experimental system has not been established so far. Here, we develop a noninvasive and unconstrained mechanochemical imaging microscopy. This microscopy enables us to evoke nano-scale nonlocalized vibrations with controllable vibration properties using a piezoelectric acoustic transducer system and quantify calcium response of a freely moving C. elegans at a single cell resolution. Using this microscopy, we clearly detected the calcium response of a single interneuron during C. elegans escape response to nano-scale vibration. Thus, this microscopy will facilitate understanding of in vivo mechanochemical physiology in the future.
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spelling pubmed-60255162018-07-09 Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans Sugi, Takuma Igarashi, Ryuji Nishimura, Masaki Materials (Basel) Article Physical forces are transduced into chemical reactions, thereby ultimately making a large impact on the whole-animal level phenotypes such as homeostasis, development and behavior. To understand mechano-chemical transduction, mechanical input should be quantitatively delivered with controllable vibration properties–frequency, amplitude and duration, and its chemical output should be noninvasively quantified in an unconstrained animal. However, such an experimental system has not been established so far. Here, we develop a noninvasive and unconstrained mechanochemical imaging microscopy. This microscopy enables us to evoke nano-scale nonlocalized vibrations with controllable vibration properties using a piezoelectric acoustic transducer system and quantify calcium response of a freely moving C. elegans at a single cell resolution. Using this microscopy, we clearly detected the calcium response of a single interneuron during C. elegans escape response to nano-scale vibration. Thus, this microscopy will facilitate understanding of in vivo mechanochemical physiology in the future. MDPI 2018-06-19 /pmc/articles/PMC6025516/ /pubmed/29921777 http://dx.doi.org/10.3390/ma11061034 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sugi, Takuma
Igarashi, Ryuji
Nishimura, Masaki
Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title_full Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title_fullStr Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title_full_unstemmed Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title_short Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
title_sort noninvasive mechanochemical imaging in unconstrained caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025516/
https://www.ncbi.nlm.nih.gov/pubmed/29921777
http://dx.doi.org/10.3390/ma11061034
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