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Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation

The high diversity of phenolic compounds (PC) found in food matrices makes it challenging to analyze their bioavailability and their impact on health and functional metabolism. It is well recognized that PC do modulate the composition of the gut microbiota (GM), however, the literature still lacks s...

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Autores principales: Chacar, Stéphanie, Tarighi, Mehrad, Fares, Nassim, Faivre, Jean-François, Louka, Nicolas, Maroun, Richard G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025592/
https://www.ncbi.nlm.nih.gov/pubmed/29866989
http://dx.doi.org/10.3390/antiox7060075
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author Chacar, Stéphanie
Tarighi, Mehrad
Fares, Nassim
Faivre, Jean-François
Louka, Nicolas
Maroun, Richard G.
author_facet Chacar, Stéphanie
Tarighi, Mehrad
Fares, Nassim
Faivre, Jean-François
Louka, Nicolas
Maroun, Richard G.
author_sort Chacar, Stéphanie
collection PubMed
description The high diversity of phenolic compounds (PC) found in food matrices makes it challenging to analyze their bioavailability and their impact on health and functional metabolism. It is well recognized that PC do modulate the composition of the gut microbiota (GM), however, the literature still lacks significant data concerning the link between the metabolic fate of the ingested compounds and their bioactivity, mainly when considering the secondary metabolites produced. In this study, we assessed the metabolic fate of PC for a period covering 14 months of daily intake to identify the metabolites that could be responsible for the effects of PC on the GM observed in our previous work. Urinary analysis of polyphenol metabolites was performed using a high resolution mass spectrometry LC-QTOF-MS method. Among the sixteen metabolites identified, 3-hydroxyphenylacetic acid and 2-(4-hydroxyphenyl) propionic acid were detected simultaneously and, therefore, correlated with the growth of Bifidobacterium in the rat GM. In addition, Daidzedin, detected only at 14 months post-treatment, mostly interfered with the growth inhibition of Clostridium (Cluster I). In conclusion, the impact of the long-term intake of PC on rat GM seems to be related to specific metabolites produced after ingestion of the parental compounds and this may also be due to their additional synergistic effects.
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spelling pubmed-60255922018-07-09 Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation Chacar, Stéphanie Tarighi, Mehrad Fares, Nassim Faivre, Jean-François Louka, Nicolas Maroun, Richard G. Antioxidants (Basel) Communication The high diversity of phenolic compounds (PC) found in food matrices makes it challenging to analyze their bioavailability and their impact on health and functional metabolism. It is well recognized that PC do modulate the composition of the gut microbiota (GM), however, the literature still lacks significant data concerning the link between the metabolic fate of the ingested compounds and their bioactivity, mainly when considering the secondary metabolites produced. In this study, we assessed the metabolic fate of PC for a period covering 14 months of daily intake to identify the metabolites that could be responsible for the effects of PC on the GM observed in our previous work. Urinary analysis of polyphenol metabolites was performed using a high resolution mass spectrometry LC-QTOF-MS method. Among the sixteen metabolites identified, 3-hydroxyphenylacetic acid and 2-(4-hydroxyphenyl) propionic acid were detected simultaneously and, therefore, correlated with the growth of Bifidobacterium in the rat GM. In addition, Daidzedin, detected only at 14 months post-treatment, mostly interfered with the growth inhibition of Clostridium (Cluster I). In conclusion, the impact of the long-term intake of PC on rat GM seems to be related to specific metabolites produced after ingestion of the parental compounds and this may also be due to their additional synergistic effects. MDPI 2018-06-04 /pmc/articles/PMC6025592/ /pubmed/29866989 http://dx.doi.org/10.3390/antiox7060075 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Chacar, Stéphanie
Tarighi, Mehrad
Fares, Nassim
Faivre, Jean-François
Louka, Nicolas
Maroun, Richard G.
Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title_full Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title_fullStr Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title_full_unstemmed Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title_short Identification of Phenolic Compounds-Rich Grape Pomace Extracts Urine Metabolites and Correlation with Gut Microbiota Modulation
title_sort identification of phenolic compounds-rich grape pomace extracts urine metabolites and correlation with gut microbiota modulation
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025592/
https://www.ncbi.nlm.nih.gov/pubmed/29866989
http://dx.doi.org/10.3390/antiox7060075
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