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Real-Time Imaging of Retinal Ganglion Cell Apoptosis

Monitoring real-time apoptosis in-vivo is an unmet need of neurodegeneration science, both in clinical and research settings. For patients, earlier diagnosis before the onset of symptoms provides a window of time in which to instigate treatment. For researchers, being able to objectively monitor the...

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Autores principales: Yap, Timothy E., Donna, Piero, Almonte, Melanie T., Cordeiro, Maria Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025611/
https://www.ncbi.nlm.nih.gov/pubmed/29914056
http://dx.doi.org/10.3390/cells7060060
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author Yap, Timothy E.
Donna, Piero
Almonte, Melanie T.
Cordeiro, Maria Francesca
author_facet Yap, Timothy E.
Donna, Piero
Almonte, Melanie T.
Cordeiro, Maria Francesca
author_sort Yap, Timothy E.
collection PubMed
description Monitoring real-time apoptosis in-vivo is an unmet need of neurodegeneration science, both in clinical and research settings. For patients, earlier diagnosis before the onset of symptoms provides a window of time in which to instigate treatment. For researchers, being able to objectively monitor the rates of underlying degenerative processes at a cellular level provides a biomarker with which to test novel therapeutics. The DARC (Detection of Apoptosing Retinal Cells) project has developed a minimally invasive method using fluorescent annexin A5 to detect rates of apoptosis in retinal ganglion cells, the key pathological process in glaucoma. Numerous animal studies have used DARC to show efficacy of novel, pressure-independent treatment strategies in models of glaucoma and other conditions where retinal apoptosis is reported, including Alzheimer’s disease. This may forge exciting new links in the clinical science of treating both cognitive and visual decline. Human trials are now underway, successfully demonstrating the safety and efficacy of the technique to differentiate patients with progressive neurodegeneration from healthy individuals. We review the current perspectives on retinal ganglion cell apoptosis, the way in which this can be imaged, and the exciting advantages that these future methods hold in store.
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spelling pubmed-60256112018-07-09 Real-Time Imaging of Retinal Ganglion Cell Apoptosis Yap, Timothy E. Donna, Piero Almonte, Melanie T. Cordeiro, Maria Francesca Cells Review Monitoring real-time apoptosis in-vivo is an unmet need of neurodegeneration science, both in clinical and research settings. For patients, earlier diagnosis before the onset of symptoms provides a window of time in which to instigate treatment. For researchers, being able to objectively monitor the rates of underlying degenerative processes at a cellular level provides a biomarker with which to test novel therapeutics. The DARC (Detection of Apoptosing Retinal Cells) project has developed a minimally invasive method using fluorescent annexin A5 to detect rates of apoptosis in retinal ganglion cells, the key pathological process in glaucoma. Numerous animal studies have used DARC to show efficacy of novel, pressure-independent treatment strategies in models of glaucoma and other conditions where retinal apoptosis is reported, including Alzheimer’s disease. This may forge exciting new links in the clinical science of treating both cognitive and visual decline. Human trials are now underway, successfully demonstrating the safety and efficacy of the technique to differentiate patients with progressive neurodegeneration from healthy individuals. We review the current perspectives on retinal ganglion cell apoptosis, the way in which this can be imaged, and the exciting advantages that these future methods hold in store. MDPI 2018-06-15 /pmc/articles/PMC6025611/ /pubmed/29914056 http://dx.doi.org/10.3390/cells7060060 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yap, Timothy E.
Donna, Piero
Almonte, Melanie T.
Cordeiro, Maria Francesca
Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title_full Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title_fullStr Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title_full_unstemmed Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title_short Real-Time Imaging of Retinal Ganglion Cell Apoptosis
title_sort real-time imaging of retinal ganglion cell apoptosis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025611/
https://www.ncbi.nlm.nih.gov/pubmed/29914056
http://dx.doi.org/10.3390/cells7060060
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