Prevalence and diversity of Rickettsia species in ectoparasites collected from small rodents in Lithuania

BACKGROUND: Rickettsiae are emerging pathogens causing public health problems in many countries around the world. Rickettsia spp. are found in association with a wide range of arthropods which feed on different species of animals. However, the distribution and natural cycle of Rickettsia species and their association with different arthropod vectors are not fully established. The aim of this study was to investigate the presence and prevalence of Rickettsia spp. in ticks, mites and fleas parasitizing different species of small mammals in Lithuania and to molecularly characterize the Rickettsia spp. obtained from different ectoparasites. RESULTS: A total of 1261 ectoparasites (596 Ixodes ricinus ticks, 550 mites of five species and 115 fleas of eight species) collected from 238 rodents in Lithuania during 2013–2014 were investigated for the presence of Rickettsia pathogens. Infection rates were calculated as the maximum likelihood estimation (MLE) with 95% confidence intervals (CI). The infection rate varied among ectoparasites and was found highest in fleas 43.5%, followed by I. ricinus ticks (MLE = 26.5%; 95% CI: 22.2–31.3%) and then mites (MLE = 9.3%; 95% CI: 7.0–12.2%). Sequence analysis of partial gltA and 17kDa genes revealed the presence of Rickettsia helvetica, R. felis, R. monacensis, Rickettsia sp. and rickettsial endosymbionts. Four Rickettsia spp. were identified in fleas, while three Rickettsia spp. were identified in Laelapidae mites and only one (R. helvetica) in I. ricinus ticks. CONCLUSIONS: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 11 species of ectoparasites of small rodents in Lithuania. The present data extend the knowledge on the distribution of Rickettsia spp. and their association with different arthropod vectors. Prior to our study, R. felis had never been identified in Lithuania. To our knowledge, this is also the first report of R. felis in L. agilis and H. microti mites and in Ct. agyrtes and H. talpae fleas, as well as the first detection of R. monacensis in Ct. agyrtes fleas. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-2947-9) contains supplementary material, which is available to authorized users.