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Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae)
PREMISE OF THE STUDY: Microsatellite markers were developed for Smilax rotundifolia (Smilacaceae), an understory vine widely distributed in eastern North America, to investigate genetic diversity and structure. Cross‐amplification was tested in three congeneric species: S. china, S. riparia, and S....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025819/ https://www.ncbi.nlm.nih.gov/pubmed/30131905 http://dx.doi.org/10.1002/aps3.1163 |
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author | Wang, Ruihong Li, Mengdi Wu, Xue Shen, Chao Yu, Wendi Liu, Jinliang Qi, Zhechen Li, Pan |
author_facet | Wang, Ruihong Li, Mengdi Wu, Xue Shen, Chao Yu, Wendi Liu, Jinliang Qi, Zhechen Li, Pan |
author_sort | Wang, Ruihong |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite markers were developed for Smilax rotundifolia (Smilacaceae), an understory vine widely distributed in eastern North America, to investigate genetic diversity and structure. Cross‐amplification was tested in three congeneric species: S. china, S. riparia, and S. walteri. METHODS AND RESULTS: A total of 6153 simple sequence repeat primer pairs were detected from the de novo–assembled transcriptome data (88,3962 contigs) of S. rotundifolia. Thirty‐three polymorphic microsatellite loci were selected for further analysis among 96 individuals representing four natural populations of the species. The number of alleles ranged from two to 15, and 87.9% of the developed primer pairs could be cross‐amplified in at least one of three congeneric Smilax species. CONCLUSIONS: The simple sequence repeat markers developed in this study will facilitate further studies on genetic diversity and phylogeographic patterns of S. rotundifolia and provide additional potential microsatellite resources for other Smilax species. |
format | Online Article Text |
id | pubmed-6025819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60258192018-08-21 Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) Wang, Ruihong Li, Mengdi Wu, Xue Shen, Chao Yu, Wendi Liu, Jinliang Qi, Zhechen Li, Pan Appl Plant Sci Primer Notes PREMISE OF THE STUDY: Microsatellite markers were developed for Smilax rotundifolia (Smilacaceae), an understory vine widely distributed in eastern North America, to investigate genetic diversity and structure. Cross‐amplification was tested in three congeneric species: S. china, S. riparia, and S. walteri. METHODS AND RESULTS: A total of 6153 simple sequence repeat primer pairs were detected from the de novo–assembled transcriptome data (88,3962 contigs) of S. rotundifolia. Thirty‐three polymorphic microsatellite loci were selected for further analysis among 96 individuals representing four natural populations of the species. The number of alleles ranged from two to 15, and 87.9% of the developed primer pairs could be cross‐amplified in at least one of three congeneric Smilax species. CONCLUSIONS: The simple sequence repeat markers developed in this study will facilitate further studies on genetic diversity and phylogeographic patterns of S. rotundifolia and provide additional potential microsatellite resources for other Smilax species. John Wiley and Sons Inc. 2018-06-26 /pmc/articles/PMC6025819/ /pubmed/30131905 http://dx.doi.org/10.1002/aps3.1163 Text en Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Primer Notes Wang, Ruihong Li, Mengdi Wu, Xue Shen, Chao Yu, Wendi Liu, Jinliang Qi, Zhechen Li, Pan Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title | Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title_full | Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title_fullStr | Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title_full_unstemmed | Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title_short | Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae) |
title_sort | characterization of polymorphic microsatellite loci for north american common greenbrier, smilax rotundifolia (smilacaceae) |
topic | Primer Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025819/ https://www.ncbi.nlm.nih.gov/pubmed/30131905 http://dx.doi.org/10.1002/aps3.1163 |
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