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Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor...

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Autores principales: Murakami, Eiichi, Cheng, Jeryl, Gysel, Kira, Bozsoki, Zoltan, Kawaharada, Yasuyuki, Hjuler, Christian Toftegaard, Sørensen, Kasper Kildegaard, Tao, Ke, Kelly, Simon, Venice, Francesco, Genre, Andrea, Thygesen, Mikkel Boas, de Jong, Noor, Vinther, Maria, Jensen, Dorthe Bødker, Jensen, Knud Jørgen, Blaise, Michael, Madsen, Lene Heegaard, Andersen, Kasper Røjkjær, Stougaard, Jens, Radutoiu, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025957/
https://www.ncbi.nlm.nih.gov/pubmed/29957177
http://dx.doi.org/10.7554/eLife.33506
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author Murakami, Eiichi
Cheng, Jeryl
Gysel, Kira
Bozsoki, Zoltan
Kawaharada, Yasuyuki
Hjuler, Christian Toftegaard
Sørensen, Kasper Kildegaard
Tao, Ke
Kelly, Simon
Venice, Francesco
Genre, Andrea
Thygesen, Mikkel Boas
de Jong, Noor
Vinther, Maria
Jensen, Dorthe Bødker
Jensen, Knud Jørgen
Blaise, Michael
Madsen, Lene Heegaard
Andersen, Kasper Røjkjær
Stougaard, Jens
Radutoiu, Simona
author_facet Murakami, Eiichi
Cheng, Jeryl
Gysel, Kira
Bozsoki, Zoltan
Kawaharada, Yasuyuki
Hjuler, Christian Toftegaard
Sørensen, Kasper Kildegaard
Tao, Ke
Kelly, Simon
Venice, Francesco
Genre, Andrea
Thygesen, Mikkel Boas
de Jong, Noor
Vinther, Maria
Jensen, Dorthe Bødker
Jensen, Knud Jørgen
Blaise, Michael
Madsen, Lene Heegaard
Andersen, Kasper Røjkjær
Stougaard, Jens
Radutoiu, Simona
author_sort Murakami, Eiichi
collection PubMed
description Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.
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spelling pubmed-60259572018-07-05 Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus Murakami, Eiichi Cheng, Jeryl Gysel, Kira Bozsoki, Zoltan Kawaharada, Yasuyuki Hjuler, Christian Toftegaard Sørensen, Kasper Kildegaard Tao, Ke Kelly, Simon Venice, Francesco Genre, Andrea Thygesen, Mikkel Boas de Jong, Noor Vinther, Maria Jensen, Dorthe Bødker Jensen, Knud Jørgen Blaise, Michael Madsen, Lene Heegaard Andersen, Kasper Røjkjær Stougaard, Jens Radutoiu, Simona eLife Plant Biology Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia. eLife Sciences Publications, Ltd 2018-06-29 /pmc/articles/PMC6025957/ /pubmed/29957177 http://dx.doi.org/10.7554/eLife.33506 Text en © 2018, Murakami et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Murakami, Eiichi
Cheng, Jeryl
Gysel, Kira
Bozsoki, Zoltan
Kawaharada, Yasuyuki
Hjuler, Christian Toftegaard
Sørensen, Kasper Kildegaard
Tao, Ke
Kelly, Simon
Venice, Francesco
Genre, Andrea
Thygesen, Mikkel Boas
de Jong, Noor
Vinther, Maria
Jensen, Dorthe Bødker
Jensen, Knud Jørgen
Blaise, Michael
Madsen, Lene Heegaard
Andersen, Kasper Røjkjær
Stougaard, Jens
Radutoiu, Simona
Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title_full Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title_fullStr Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title_full_unstemmed Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title_short Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
title_sort epidermal lysm receptor ensures robust symbiotic signalling in lotus japonicus
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025957/
https://www.ncbi.nlm.nih.gov/pubmed/29957177
http://dx.doi.org/10.7554/eLife.33506
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