A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct...

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Detalles Bibliográficos
Autores principales: Septiadi, D., Bourquin, J., Durantie, E., Petri-Fink, A., Rothen-Rutishauser, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026119/
https://www.ncbi.nlm.nih.gov/pubmed/29959370
http://dx.doi.org/10.1038/s41598-018-28206-2
Descripción
Sumario:Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.

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