A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct...

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Autores principales: Septiadi, D., Bourquin, J., Durantie, E., Petri-Fink, A., Rothen-Rutishauser, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026119/
https://www.ncbi.nlm.nih.gov/pubmed/29959370
http://dx.doi.org/10.1038/s41598-018-28206-2
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author Septiadi, D.
Bourquin, J.
Durantie, E.
Petri-Fink, A.
Rothen-Rutishauser, B.
author_facet Septiadi, D.
Bourquin, J.
Durantie, E.
Petri-Fink, A.
Rothen-Rutishauser, B.
author_sort Septiadi, D.
collection PubMed
description Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.
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spelling pubmed-60261192018-07-09 A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures Septiadi, D. Bourquin, J. Durantie, E. Petri-Fink, A. Rothen-Rutishauser, B. Sci Rep Article Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes. Nature Publishing Group UK 2018-06-29 /pmc/articles/PMC6026119/ /pubmed/29959370 http://dx.doi.org/10.1038/s41598-018-28206-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Septiadi, D.
Bourquin, J.
Durantie, E.
Petri-Fink, A.
Rothen-Rutishauser, B.
A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title_full A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title_fullStr A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title_full_unstemmed A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title_short A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
title_sort novel sample holder for 4d live cell imaging to study cellular dynamics in complex 3d tissue cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026119/
https://www.ncbi.nlm.nih.gov/pubmed/29959370
http://dx.doi.org/10.1038/s41598-018-28206-2
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