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Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates

Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regen...

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Autores principales: Wang, Yi-Jing, Zhao, Pan, Sui, Bing-Dong, Liu, Nu, Hu, Cheng-Hu, Chen, Ji, Zheng, Chen-Xi, Liu, An-Qi, Xuan, Kun, Pan, Ya-Ping, Jin, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026147/
https://www.ncbi.nlm.nih.gov/pubmed/29959311
http://dx.doi.org/10.1038/s12276-018-0109-y
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author Wang, Yi-Jing
Zhao, Pan
Sui, Bing-Dong
Liu, Nu
Hu, Cheng-Hu
Chen, Ji
Zheng, Chen-Xi
Liu, An-Qi
Xuan, Kun
Pan, Ya-Ping
Jin, Yan
author_facet Wang, Yi-Jing
Zhao, Pan
Sui, Bing-Dong
Liu, Nu
Hu, Cheng-Hu
Chen, Ji
Zheng, Chen-Xi
Liu, An-Qi
Xuan, Kun
Pan, Ya-Ping
Jin, Yan
author_sort Wang, Yi-Jing
collection PubMed
description Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine.
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spelling pubmed-60261472018-07-11 Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates Wang, Yi-Jing Zhao, Pan Sui, Bing-Dong Liu, Nu Hu, Cheng-Hu Chen, Ji Zheng, Chen-Xi Liu, An-Qi Xuan, Kun Pan, Ya-Ping Jin, Yan Exp Mol Med Article Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine. Nature Publishing Group UK 2018-06-27 /pmc/articles/PMC6026147/ /pubmed/29959311 http://dx.doi.org/10.1038/s12276-018-0109-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Yi-Jing
Zhao, Pan
Sui, Bing-Dong
Liu, Nu
Hu, Cheng-Hu
Chen, Ji
Zheng, Chen-Xi
Liu, An-Qi
Xuan, Kun
Pan, Ya-Ping
Jin, Yan
Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title_full Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title_fullStr Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title_full_unstemmed Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title_short Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
title_sort resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026147/
https://www.ncbi.nlm.nih.gov/pubmed/29959311
http://dx.doi.org/10.1038/s12276-018-0109-y
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