Cargando…
Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract
BACKGROUND: The antioxidant effects of Panax ginseng have been reported in several articles; however, little is known about the antimelanogenesis effect, skin-protective effect, and cellular mechanism of Panax ginseng, especially of P. ginseng calyx. To understand how an ethanol extract of P. ginsen...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026384/ https://www.ncbi.nlm.nih.gov/pubmed/29983620 http://dx.doi.org/10.1016/j.jgr.2018.02.007 |
_version_ | 1783336430918959104 |
---|---|
author | Lee, Jeong-Ook Kim, Eunji Kim, Ji Hye Hong, Yo Han Kim, Han Gyung Jeong, Deok Kim, Juewon Kim, Su Hwan Park, Chanwoong Seo, Dae Bang Son, Young-Jin Han, Sang Yun Cho, Jae Youl |
author_facet | Lee, Jeong-Ook Kim, Eunji Kim, Ji Hye Hong, Yo Han Kim, Han Gyung Jeong, Deok Kim, Juewon Kim, Su Hwan Park, Chanwoong Seo, Dae Bang Son, Young-Jin Han, Sang Yun Cho, Jae Youl |
author_sort | Lee, Jeong-Ook |
collection | PubMed |
description | BACKGROUND: The antioxidant effects of Panax ginseng have been reported in several articles; however, little is known about the antimelanogenesis effect, skin-protective effect, and cellular mechanism of Panax ginseng, especially of P. ginseng calyx. To understand how an ethanol extract of P. ginseng berry calyx (Pg-C-EE) exerts skin-protective effects, we studied its activities in activated melanocytes and reactive oxygen species (ROS)–induced keratinocytes. METHODS: To confirm the antimelanogenesis effect of Pg-C-EE, we analyzed melanin synthesis and secretion and messenger RNA and protein expression levels of related genes. Ultraviolet B (UVB) and hydrogen peroxide (H(2)O(2)) were used to induce cell damage by ROS generation. To examine whether this damage is inhibited by Pg-C-EE, we performed cell viability assays and gene expression and transcriptional activation analyses. RESULTS: Pg-C-EE inhibited melanin synthesis and secretion by blocking activator protein 1 regulatory enzymes such as p38, extracellular signal-regulated kinases (ERKs), and cyclic adenosine monophosphate response element–binding protein. Pg-C-EE also suppressed ROS generation induced by H(2)O(2) and UVB. Treatment with Pg-C-EE decreased the expression of matrix metalloproteinases, mitogen-activated protein kinases, and hyaluronidases and increased the cell survival rate. CONCLUSION: These results suggest that Pg-C-EE may have antimelanogenesis properties and skin-protective properties through regulation of activator protein 1 and cyclic adenosine monophosphate response element–binding protein signaling. Pg-C-EE may be used as a skin-improving agent, with moisture retention and whitening effects. |
format | Online Article Text |
id | pubmed-6026384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-60263842018-07-06 Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract Lee, Jeong-Ook Kim, Eunji Kim, Ji Hye Hong, Yo Han Kim, Han Gyung Jeong, Deok Kim, Juewon Kim, Su Hwan Park, Chanwoong Seo, Dae Bang Son, Young-Jin Han, Sang Yun Cho, Jae Youl J Ginseng Res Research Article BACKGROUND: The antioxidant effects of Panax ginseng have been reported in several articles; however, little is known about the antimelanogenesis effect, skin-protective effect, and cellular mechanism of Panax ginseng, especially of P. ginseng calyx. To understand how an ethanol extract of P. ginseng berry calyx (Pg-C-EE) exerts skin-protective effects, we studied its activities in activated melanocytes and reactive oxygen species (ROS)–induced keratinocytes. METHODS: To confirm the antimelanogenesis effect of Pg-C-EE, we analyzed melanin synthesis and secretion and messenger RNA and protein expression levels of related genes. Ultraviolet B (UVB) and hydrogen peroxide (H(2)O(2)) were used to induce cell damage by ROS generation. To examine whether this damage is inhibited by Pg-C-EE, we performed cell viability assays and gene expression and transcriptional activation analyses. RESULTS: Pg-C-EE inhibited melanin synthesis and secretion by blocking activator protein 1 regulatory enzymes such as p38, extracellular signal-regulated kinases (ERKs), and cyclic adenosine monophosphate response element–binding protein. Pg-C-EE also suppressed ROS generation induced by H(2)O(2) and UVB. Treatment with Pg-C-EE decreased the expression of matrix metalloproteinases, mitogen-activated protein kinases, and hyaluronidases and increased the cell survival rate. CONCLUSION: These results suggest that Pg-C-EE may have antimelanogenesis properties and skin-protective properties through regulation of activator protein 1 and cyclic adenosine monophosphate response element–binding protein signaling. Pg-C-EE may be used as a skin-improving agent, with moisture retention and whitening effects. Elsevier 2018-07 2018-02-21 /pmc/articles/PMC6026384/ /pubmed/29983620 http://dx.doi.org/10.1016/j.jgr.2018.02.007 Text en © 2018 The Korean Society of Ginseng, Published by Elsevier Korea LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Lee, Jeong-Ook Kim, Eunji Kim, Ji Hye Hong, Yo Han Kim, Han Gyung Jeong, Deok Kim, Juewon Kim, Su Hwan Park, Chanwoong Seo, Dae Bang Son, Young-Jin Han, Sang Yun Cho, Jae Youl Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title | Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title_full | Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title_fullStr | Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title_full_unstemmed | Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title_short | Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract |
title_sort | antimelanogenesis and skin-protective activities of panax ginseng calyx ethanol extract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026384/ https://www.ncbi.nlm.nih.gov/pubmed/29983620 http://dx.doi.org/10.1016/j.jgr.2018.02.007 |
work_keys_str_mv | AT leejeongook antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT kimeunji antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT kimjihye antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT hongyohan antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT kimhangyung antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT jeongdeok antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT kimjuewon antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT kimsuhwan antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT parkchanwoong antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT seodaebang antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT sonyoungjin antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT hansangyun antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract AT chojaeyoul antimelanogenesisandskinprotectiveactivitiesofpanaxginsengcalyxethanolextract |