The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress

The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic stresses. Here, the isolation of CmCPL1, a chrysanthemum (Chrysanthemum morifolium) gene encoding this enzyme is described. Its predicted 955 residue gene product includes the FC...

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Autores principales: Qi, Yuying, Liu, Yanan, Zhang, Zixin, Gao, Jiaojiao, Guan, Zhiyong, Fang, Weimin, Chen, Sumei, Chen, Fadi, Jiang, Jiafu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026497/
https://www.ncbi.nlm.nih.gov/pubmed/29977573
http://dx.doi.org/10.1038/s41438-018-0037-y
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author Qi, Yuying
Liu, Yanan
Zhang, Zixin
Gao, Jiaojiao
Guan, Zhiyong
Fang, Weimin
Chen, Sumei
Chen, Fadi
Jiang, Jiafu
author_facet Qi, Yuying
Liu, Yanan
Zhang, Zixin
Gao, Jiaojiao
Guan, Zhiyong
Fang, Weimin
Chen, Sumei
Chen, Fadi
Jiang, Jiafu
author_sort Qi, Yuying
collection PubMed
description The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic stresses. Here, the isolation of CmCPL1, a chrysanthemum (Chrysanthemum morifolium) gene encoding this enzyme is described. Its predicted 955 residue gene product includes the FCPH catalytic domain, two double-stranded RNA binding motifs, and a nuclear localization signal. A sub-cellular localization assay confirmed that CmCPL1 was expressed in the nucleus. CmCPL1 transcription was shown to be significantly inducible by heat stress. The over-expression and knockdown of CmCPL1, respectively, increased and diminished the tolerance of chrysanthemum to heat stress, which maybe dependent on the regulation of CmCPL1 and on the expression of downstream heat stress-responsive genes.
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spelling pubmed-60264972018-07-05 The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress Qi, Yuying Liu, Yanan Zhang, Zixin Gao, Jiaojiao Guan, Zhiyong Fang, Weimin Chen, Sumei Chen, Fadi Jiang, Jiafu Hortic Res Article The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic stresses. Here, the isolation of CmCPL1, a chrysanthemum (Chrysanthemum morifolium) gene encoding this enzyme is described. Its predicted 955 residue gene product includes the FCPH catalytic domain, two double-stranded RNA binding motifs, and a nuclear localization signal. A sub-cellular localization assay confirmed that CmCPL1 was expressed in the nucleus. CmCPL1 transcription was shown to be significantly inducible by heat stress. The over-expression and knockdown of CmCPL1, respectively, increased and diminished the tolerance of chrysanthemum to heat stress, which maybe dependent on the regulation of CmCPL1 and on the expression of downstream heat stress-responsive genes. Nature Publishing Group UK 2018-07-01 /pmc/articles/PMC6026497/ /pubmed/29977573 http://dx.doi.org/10.1038/s41438-018-0037-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Qi, Yuying
Liu, Yanan
Zhang, Zixin
Gao, Jiaojiao
Guan, Zhiyong
Fang, Weimin
Chen, Sumei
Chen, Fadi
Jiang, Jiafu
The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title_full The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title_fullStr The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title_full_unstemmed The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title_short The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress
title_sort over-expression of a chrysanthemum gene encoding an rna polymerase ii ctd phosphatase-like 1 enzyme enhances tolerance to heat stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026497/
https://www.ncbi.nlm.nih.gov/pubmed/29977573
http://dx.doi.org/10.1038/s41438-018-0037-y
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