Cargando…
Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model
BACKGROUND: The current study was designed to explore the pathway through which adipose-derived mesenchymal stem cells (ADMSCs) affect brain ischemic injury. MATERIAL/METHODS: The improving effect of ADMSCs on the brain function and structure was evaluated in a middle cerebral artery occlusion (MCAO...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026597/ https://www.ncbi.nlm.nih.gov/pubmed/29888735 http://dx.doi.org/10.12659/MSM.907096 |
_version_ | 1783336464288841728 |
---|---|
author | Chi, Lumei Huang, Yujing Mao, Ying Wu, Kunjun Zhang, Li Nan, Guangxian |
author_facet | Chi, Lumei Huang, Yujing Mao, Ying Wu, Kunjun Zhang, Li Nan, Guangxian |
author_sort | Chi, Lumei |
collection | PubMed |
description | BACKGROUND: The current study was designed to explore the pathway through which adipose-derived mesenchymal stem cells (ADMSCs) affect brain ischemic injury. MATERIAL/METHODS: The improving effect of ADMSCs on the brain function and structure was evaluated in a middle cerebral artery occlusion (MCAO) rat model. The permeability of the brain-blood barrier (BBB), inflammatory response, and endoplasmic reticulum (ER) stress-related signaling induced by ischemia were determined. RESULTS: The administration of ADMSCs decreased neurological severity score when compared with that in the MCAO group and also restricted the brain infarction area as well as cell apoptosis. ADMSCs suppressed the inflammation in brains by decreasing the expressions of IL-1β, IL-6, and TNF-α, contributing to the decreased permeability of the BBB. The expressions of pro-apoptosis factors in ER stress were inhibited while that of anti-apoptosis factors were induced. CONCLUSIONS: ADMSCs affected brain injury in multiple ways, not only by suppressing inflammation in the brain infarction area, but also by blocking ER stress-induced apoptosis. |
format | Online Article Text |
id | pubmed-6026597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60265972018-07-09 Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model Chi, Lumei Huang, Yujing Mao, Ying Wu, Kunjun Zhang, Li Nan, Guangxian Med Sci Monit Animal Study BACKGROUND: The current study was designed to explore the pathway through which adipose-derived mesenchymal stem cells (ADMSCs) affect brain ischemic injury. MATERIAL/METHODS: The improving effect of ADMSCs on the brain function and structure was evaluated in a middle cerebral artery occlusion (MCAO) rat model. The permeability of the brain-blood barrier (BBB), inflammatory response, and endoplasmic reticulum (ER) stress-related signaling induced by ischemia were determined. RESULTS: The administration of ADMSCs decreased neurological severity score when compared with that in the MCAO group and also restricted the brain infarction area as well as cell apoptosis. ADMSCs suppressed the inflammation in brains by decreasing the expressions of IL-1β, IL-6, and TNF-α, contributing to the decreased permeability of the BBB. The expressions of pro-apoptosis factors in ER stress were inhibited while that of anti-apoptosis factors were induced. CONCLUSIONS: ADMSCs affected brain injury in multiple ways, not only by suppressing inflammation in the brain infarction area, but also by blocking ER stress-induced apoptosis. International Scientific Literature, Inc. 2018-06-11 /pmc/articles/PMC6026597/ /pubmed/29888735 http://dx.doi.org/10.12659/MSM.907096 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Chi, Lumei Huang, Yujing Mao, Ying Wu, Kunjun Zhang, Li Nan, Guangxian Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title | Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title_full | Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title_fullStr | Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title_full_unstemmed | Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title_short | Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model |
title_sort | tail vein infusion of adipose-derived mesenchymal stem cell alleviated inflammatory response and improved blood brain barrier condition by suppressing endoplasmic reticulum stress in a middle cerebral artery occlusion rat model |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026597/ https://www.ncbi.nlm.nih.gov/pubmed/29888735 http://dx.doi.org/10.12659/MSM.907096 |
work_keys_str_mv | AT chilumei tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel AT huangyujing tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel AT maoying tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel AT wukunjun tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel AT zhangli tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel AT nanguangxian tailveininfusionofadiposederivedmesenchymalstemcellalleviatedinflammatoryresponseandimprovedbloodbrainbarrierconditionbysuppressingendoplasmicreticulumstressinamiddlecerebralarteryocclusionratmodel |