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Microfluidic enrichment of plasma cells improves treatment of multiple myeloma

Cytogenetic alterations form the basis for risk stratification for multiple myeloma (MM) and guide the selection of therapy; however, current pathology assays performed on bone marrow samples can produce false‐negatives due to the unpredictable distribution and rarity of MM cells. Here, we report on...

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Autores principales: Zeng, Yunjing, Gao, Li, Luo, Xiaoqing, Chen, Yan, Kabeer, Mustafa H., Chen, Xuelian, Stucky, Andres, Loudon, William G., Li, Shengwen C., Zhang, Xi, Zhong, Jiang F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026869/
https://www.ncbi.nlm.nih.gov/pubmed/29638042
http://dx.doi.org/10.1002/1878-0261.12201
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author Zeng, Yunjing
Gao, Li
Luo, Xiaoqing
Chen, Yan
Kabeer, Mustafa H.
Chen, Xuelian
Stucky, Andres
Loudon, William G.
Li, Shengwen C.
Zhang, Xi
Zhong, Jiang F.
author_facet Zeng, Yunjing
Gao, Li
Luo, Xiaoqing
Chen, Yan
Kabeer, Mustafa H.
Chen, Xuelian
Stucky, Andres
Loudon, William G.
Li, Shengwen C.
Zhang, Xi
Zhong, Jiang F.
author_sort Zeng, Yunjing
collection PubMed
description Cytogenetic alterations form the basis for risk stratification for multiple myeloma (MM) and guide the selection of therapy; however, current pathology assays performed on bone marrow samples can produce false‐negatives due to the unpredictable distribution and rarity of MM cells. Here, we report on a microfluidic device used to facilitate CD45 depletion to enhance the detection of cytogenetic alterations in plasma cells (PCs). Bone marrow samples from 48 patients with MM were each divided into two aliquots. One aliquot was subjected to classic flow cytometry and fluorescent in situ hybridization (FISH). The other first went through CD45(+) cell depletion, further enriched by microfluidic size selection. The enriched samples were then analyzed using flow cytometry and FISH and compared to those analyzed using the classic method only. Unlike the traditional method, the microfluidic device removed the CD45(+) leukocytes and specifically selected PCs from the remaining white blood cells. Therefore, the microfluidic method (MF‐CD45‐TACs) significantly increased the percentage of CD38(+)/CD138(+) cells to 37.7 ± 20.4% (P < 0.001) from 10.3 ± 8.5% in bone marrow. After the MF‐CD45‐TAC enrichment, the detection rate of IgH rearrangement, del(13q14), del(17p), and 1q21 gains, rose to 56.3% (P < 0.001), 37.5% (P < 0.001), 22.9% (P < 0.001), and 41.7% (P = 0.001), respectively; all rates of detection were significantly increased compared to the classically analyzed samples. In this clinical trial, this microfluidic‐assisted assay provided a precise detection of cytogenetic alterations in PCs and improved clinical outcomes.
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spelling pubmed-60268692018-07-09 Microfluidic enrichment of plasma cells improves treatment of multiple myeloma Zeng, Yunjing Gao, Li Luo, Xiaoqing Chen, Yan Kabeer, Mustafa H. Chen, Xuelian Stucky, Andres Loudon, William G. Li, Shengwen C. Zhang, Xi Zhong, Jiang F. Mol Oncol Research Articles Cytogenetic alterations form the basis for risk stratification for multiple myeloma (MM) and guide the selection of therapy; however, current pathology assays performed on bone marrow samples can produce false‐negatives due to the unpredictable distribution and rarity of MM cells. Here, we report on a microfluidic device used to facilitate CD45 depletion to enhance the detection of cytogenetic alterations in plasma cells (PCs). Bone marrow samples from 48 patients with MM were each divided into two aliquots. One aliquot was subjected to classic flow cytometry and fluorescent in situ hybridization (FISH). The other first went through CD45(+) cell depletion, further enriched by microfluidic size selection. The enriched samples were then analyzed using flow cytometry and FISH and compared to those analyzed using the classic method only. Unlike the traditional method, the microfluidic device removed the CD45(+) leukocytes and specifically selected PCs from the remaining white blood cells. Therefore, the microfluidic method (MF‐CD45‐TACs) significantly increased the percentage of CD38(+)/CD138(+) cells to 37.7 ± 20.4% (P < 0.001) from 10.3 ± 8.5% in bone marrow. After the MF‐CD45‐TAC enrichment, the detection rate of IgH rearrangement, del(13q14), del(17p), and 1q21 gains, rose to 56.3% (P < 0.001), 37.5% (P < 0.001), 22.9% (P < 0.001), and 41.7% (P = 0.001), respectively; all rates of detection were significantly increased compared to the classically analyzed samples. In this clinical trial, this microfluidic‐assisted assay provided a precise detection of cytogenetic alterations in PCs and improved clinical outcomes. John Wiley and Sons Inc. 2018-05-12 2018-06 /pmc/articles/PMC6026869/ /pubmed/29638042 http://dx.doi.org/10.1002/1878-0261.12201 Text en © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zeng, Yunjing
Gao, Li
Luo, Xiaoqing
Chen, Yan
Kabeer, Mustafa H.
Chen, Xuelian
Stucky, Andres
Loudon, William G.
Li, Shengwen C.
Zhang, Xi
Zhong, Jiang F.
Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title_full Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title_fullStr Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title_full_unstemmed Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title_short Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
title_sort microfluidic enrichment of plasma cells improves treatment of multiple myeloma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026869/
https://www.ncbi.nlm.nih.gov/pubmed/29638042
http://dx.doi.org/10.1002/1878-0261.12201
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