Cargando…

High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens

Ibuprofen (IBU-H), a widely used anti-inflammatory, also shows a marked antimicrobial effect against several bacterial species, including those involved in cystic fibrosis such as Pseudomona aeruginosa, methicillin resistant Staphylococcus aureus and Burkholderia cepacia complex. Additionally, our r...

Descripción completa

Detalles Bibliográficos
Autores principales: Muñoz, Adrián J., Alasino, Roxana V., Garro, Ariel G., Heredia, Valeria, García, Néstor H., Cremonezzi, David C., Beltramo, Dante M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026927/
https://www.ncbi.nlm.nih.gov/pubmed/29772761
http://dx.doi.org/10.3390/ph11020047
_version_ 1783336527547334656
author Muñoz, Adrián J.
Alasino, Roxana V.
Garro, Ariel G.
Heredia, Valeria
García, Néstor H.
Cremonezzi, David C.
Beltramo, Dante M.
author_facet Muñoz, Adrián J.
Alasino, Roxana V.
Garro, Ariel G.
Heredia, Valeria
García, Néstor H.
Cremonezzi, David C.
Beltramo, Dante M.
author_sort Muñoz, Adrián J.
collection PubMed
description Ibuprofen (IBU-H), a widely used anti-inflammatory, also shows a marked antimicrobial effect against several bacterial species, including those involved in cystic fibrosis such as Pseudomona aeruginosa, methicillin resistant Staphylococcus aureus and Burkholderia cepacia complex. Additionally, our results show significant synergy between water soluble Na-ibuprofen (IBU-Na) and ionic strength. Salt concentrations above 0.5 M modify the zeta potential promoting the action of Na-IBU; thus, with 1 M sodium chloride, IBU-Na is ten times more efficient than in the absence of ionic strength, and the minimum effective contact time is reduced from hours to minutes. In short time periods, where neither IBU-Na nor controls with 1 M NaCl show activity, the combination of both leads to a reduction in the bacterial load. We also analyzed whether the changes caused by salt on the bacterial membrane also promoted the activity of other microbicide compounds used in cystic fibrosis like gentamicin, tobramycin and phosphomycin. The results show that the presence of ionic strength only enhanced the bactericidal activity of the amphipathic molecule of IBU-Na. In this respect, the effect of saline concentration was also reflected in the surface properties of IBU-Na, where, in addition to the clear differences observed between 145 mM and 1 M, singular behaviors were also found, different in each condition. The combination of anti-inflammatory activity and this improved bactericidal effect of Na-IBU in hypertonic solution provides a new alternative for the treatment of respiratory infections of fibrotic patients based on known and widely used compounds.
format Online
Article
Text
id pubmed-6026927
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60269272018-07-13 High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens Muñoz, Adrián J. Alasino, Roxana V. Garro, Ariel G. Heredia, Valeria García, Néstor H. Cremonezzi, David C. Beltramo, Dante M. Pharmaceuticals (Basel) Article Ibuprofen (IBU-H), a widely used anti-inflammatory, also shows a marked antimicrobial effect against several bacterial species, including those involved in cystic fibrosis such as Pseudomona aeruginosa, methicillin resistant Staphylococcus aureus and Burkholderia cepacia complex. Additionally, our results show significant synergy between water soluble Na-ibuprofen (IBU-Na) and ionic strength. Salt concentrations above 0.5 M modify the zeta potential promoting the action of Na-IBU; thus, with 1 M sodium chloride, IBU-Na is ten times more efficient than in the absence of ionic strength, and the minimum effective contact time is reduced from hours to minutes. In short time periods, where neither IBU-Na nor controls with 1 M NaCl show activity, the combination of both leads to a reduction in the bacterial load. We also analyzed whether the changes caused by salt on the bacterial membrane also promoted the activity of other microbicide compounds used in cystic fibrosis like gentamicin, tobramycin and phosphomycin. The results show that the presence of ionic strength only enhanced the bactericidal activity of the amphipathic molecule of IBU-Na. In this respect, the effect of saline concentration was also reflected in the surface properties of IBU-Na, where, in addition to the clear differences observed between 145 mM and 1 M, singular behaviors were also found, different in each condition. The combination of anti-inflammatory activity and this improved bactericidal effect of Na-IBU in hypertonic solution provides a new alternative for the treatment of respiratory infections of fibrotic patients based on known and widely used compounds. MDPI 2018-05-17 /pmc/articles/PMC6026927/ /pubmed/29772761 http://dx.doi.org/10.3390/ph11020047 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muñoz, Adrián J.
Alasino, Roxana V.
Garro, Ariel G.
Heredia, Valeria
García, Néstor H.
Cremonezzi, David C.
Beltramo, Dante M.
High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title_full High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title_fullStr High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title_full_unstemmed High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title_short High Concentrations of Sodium Chloride Improve Microbicidal Activity of Ibuprofen against Common Cystic Fibrosis Pathogens
title_sort high concentrations of sodium chloride improve microbicidal activity of ibuprofen against common cystic fibrosis pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026927/
https://www.ncbi.nlm.nih.gov/pubmed/29772761
http://dx.doi.org/10.3390/ph11020047
work_keys_str_mv AT munozadrianj highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT alasinoroxanav highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT garroarielg highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT herediavaleria highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT garcianestorh highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT cremonezzidavidc highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens
AT beltramodantem highconcentrationsofsodiumchlorideimprovemicrobicidalactivityofibuprofenagainstcommoncysticfibrosispathogens