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Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei

Membrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such cont...

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Detalles Bibliográficos
Autores principales: Ramakrishnan, Srinivasan, Asady, Beejan, Docampo, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027259/
https://www.ncbi.nlm.nih.gov/pubmed/29565282
http://dx.doi.org/10.3390/pathogens7020033
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author Ramakrishnan, Srinivasan
Asady, Beejan
Docampo, Roberto
author_facet Ramakrishnan, Srinivasan
Asady, Beejan
Docampo, Roberto
author_sort Ramakrishnan, Srinivasan
collection PubMed
description Membrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such contact sites in Trypanosoma brucei. In mammalian cells, endoplasmic reticulum-mitochondria contact sites facilitate mitochondrial uptake of Ca(2+) released by the ER-located inositol 1,4,5-trisphosphate receptor (InsP(3)R). However, the InsP(3)R in trypanosomes localizes to acidocalcisomes, which serve as major Ca(2+) stores in these parasites. In this work, we have used super-resolution structured illumination microscopy and electron microscopy to identify membrane contact sites that exist between acidocalcisomes and mitochondria. Furthermore, we have confirmed the close association of these organelles using proximity ligation assays. Characterization of these contact sites may be a necessary starting point towards unraveling the role of Ca(2+) in regulating trypanosome bioenergetics.
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spelling pubmed-60272592018-07-13 Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei Ramakrishnan, Srinivasan Asady, Beejan Docampo, Roberto Pathogens Article Membrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such contact sites in Trypanosoma brucei. In mammalian cells, endoplasmic reticulum-mitochondria contact sites facilitate mitochondrial uptake of Ca(2+) released by the ER-located inositol 1,4,5-trisphosphate receptor (InsP(3)R). However, the InsP(3)R in trypanosomes localizes to acidocalcisomes, which serve as major Ca(2+) stores in these parasites. In this work, we have used super-resolution structured illumination microscopy and electron microscopy to identify membrane contact sites that exist between acidocalcisomes and mitochondria. Furthermore, we have confirmed the close association of these organelles using proximity ligation assays. Characterization of these contact sites may be a necessary starting point towards unraveling the role of Ca(2+) in regulating trypanosome bioenergetics. MDPI 2018-03-22 /pmc/articles/PMC6027259/ /pubmed/29565282 http://dx.doi.org/10.3390/pathogens7020033 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramakrishnan, Srinivasan
Asady, Beejan
Docampo, Roberto
Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title_full Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title_fullStr Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title_full_unstemmed Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title_short Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
title_sort acidocalcisome-mitochondrion membrane contact sites in trypanosoma brucei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027259/
https://www.ncbi.nlm.nih.gov/pubmed/29565282
http://dx.doi.org/10.3390/pathogens7020033
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