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Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027270/ https://www.ncbi.nlm.nih.gov/pubmed/29617342 http://dx.doi.org/10.3390/pathogens7020037 |
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author | Deol, Pallavi Kattoor, Jobin Jose Sircar, Shubhankar Batra, Munish Dhama, Kuldeep Malik, Yashpal Singh |
author_facet | Deol, Pallavi Kattoor, Jobin Jose Sircar, Shubhankar Batra, Munish Dhama, Kuldeep Malik, Yashpal Singh |
author_sort | Deol, Pallavi |
collection | PubMed |
description | Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a RV-D isolate (UKD48) obtained from Uttarakhand state was analyzed. Phylogenetically, the RV-D isolate was found to be closely related to a South Korean strain, and the nucleotide percent identity varied from 80.4–84.2% with other RV-D strains available globally. Furthermore, domain investigation within 21 aligned amino acid sequences of the VP7 gene affirmed that this gene has several domains: a conserved glycosylation site (N–I–T) having an important role in protein folding; a N-terminal signal peptide (“ITG”) for endoplasmic reticulum retention; and two hydrophobic sites for elucidating transmembrane portions, antigenic structures, and so forth. The findings suggest that the VP7 gene of the Indian RV-D isolate is genetically distinct from those of other avian RV-Ds. Although biological evidence is still needed to prove the functional characteristics of these domains in outer capsid structural proteins, the present study adds new knowledge and derives the need for further investigation. |
format | Online Article Text |
id | pubmed-6027270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60272702018-07-13 Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 Deol, Pallavi Kattoor, Jobin Jose Sircar, Shubhankar Batra, Munish Dhama, Kuldeep Malik, Yashpal Singh Pathogens Brief Report Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a RV-D isolate (UKD48) obtained from Uttarakhand state was analyzed. Phylogenetically, the RV-D isolate was found to be closely related to a South Korean strain, and the nucleotide percent identity varied from 80.4–84.2% with other RV-D strains available globally. Furthermore, domain investigation within 21 aligned amino acid sequences of the VP7 gene affirmed that this gene has several domains: a conserved glycosylation site (N–I–T) having an important role in protein folding; a N-terminal signal peptide (“ITG”) for endoplasmic reticulum retention; and two hydrophobic sites for elucidating transmembrane portions, antigenic structures, and so forth. The findings suggest that the VP7 gene of the Indian RV-D isolate is genetically distinct from those of other avian RV-Ds. Although biological evidence is still needed to prove the functional characteristics of these domains in outer capsid structural proteins, the present study adds new knowledge and derives the need for further investigation. MDPI 2018-04-04 /pmc/articles/PMC6027270/ /pubmed/29617342 http://dx.doi.org/10.3390/pathogens7020037 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Deol, Pallavi Kattoor, Jobin Jose Sircar, Shubhankar Batra, Munish Dhama, Kuldeep Malik, Yashpal Singh Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title | Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title_full | Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title_fullStr | Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title_full_unstemmed | Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title_short | Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 |
title_sort | identification and in silico characterization of a genetically distinct avian rotavirus d capsid gene, vp7 |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027270/ https://www.ncbi.nlm.nih.gov/pubmed/29617342 http://dx.doi.org/10.3390/pathogens7020037 |
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