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Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7

Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a...

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Autores principales: Deol, Pallavi, Kattoor, Jobin Jose, Sircar, Shubhankar, Batra, Munish, Dhama, Kuldeep, Malik, Yashpal Singh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027270/
https://www.ncbi.nlm.nih.gov/pubmed/29617342
http://dx.doi.org/10.3390/pathogens7020037
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author Deol, Pallavi
Kattoor, Jobin Jose
Sircar, Shubhankar
Batra, Munish
Dhama, Kuldeep
Malik, Yashpal Singh
author_facet Deol, Pallavi
Kattoor, Jobin Jose
Sircar, Shubhankar
Batra, Munish
Dhama, Kuldeep
Malik, Yashpal Singh
author_sort Deol, Pallavi
collection PubMed
description Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a RV-D isolate (UKD48) obtained from Uttarakhand state was analyzed. Phylogenetically, the RV-D isolate was found to be closely related to a South Korean strain, and the nucleotide percent identity varied from 80.4–84.2% with other RV-D strains available globally. Furthermore, domain investigation within 21 aligned amino acid sequences of the VP7 gene affirmed that this gene has several domains: a conserved glycosylation site (N–I–T) having an important role in protein folding; a N-terminal signal peptide (“ITG”) for endoplasmic reticulum retention; and two hydrophobic sites for elucidating transmembrane portions, antigenic structures, and so forth. The findings suggest that the VP7 gene of the Indian RV-D isolate is genetically distinct from those of other avian RV-Ds. Although biological evidence is still needed to prove the functional characteristics of these domains in outer capsid structural proteins, the present study adds new knowledge and derives the need for further investigation.
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spelling pubmed-60272702018-07-13 Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7 Deol, Pallavi Kattoor, Jobin Jose Sircar, Shubhankar Batra, Munish Dhama, Kuldeep Malik, Yashpal Singh Pathogens Brief Report Rotavirus D (RV-D) is gaining importance as a cause of gastroenteritis and runting and stunting syndrome (RSS) in poultry. To date, information is scarce on the molecular analysis of RV-D isolates worldwide. In this study, the VP7 gene, a major constituent of outer capsid structural protein, from a RV-D isolate (UKD48) obtained from Uttarakhand state was analyzed. Phylogenetically, the RV-D isolate was found to be closely related to a South Korean strain, and the nucleotide percent identity varied from 80.4–84.2% with other RV-D strains available globally. Furthermore, domain investigation within 21 aligned amino acid sequences of the VP7 gene affirmed that this gene has several domains: a conserved glycosylation site (N–I–T) having an important role in protein folding; a N-terminal signal peptide (“ITG”) for endoplasmic reticulum retention; and two hydrophobic sites for elucidating transmembrane portions, antigenic structures, and so forth. The findings suggest that the VP7 gene of the Indian RV-D isolate is genetically distinct from those of other avian RV-Ds. Although biological evidence is still needed to prove the functional characteristics of these domains in outer capsid structural proteins, the present study adds new knowledge and derives the need for further investigation. MDPI 2018-04-04 /pmc/articles/PMC6027270/ /pubmed/29617342 http://dx.doi.org/10.3390/pathogens7020037 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Deol, Pallavi
Kattoor, Jobin Jose
Sircar, Shubhankar
Batra, Munish
Dhama, Kuldeep
Malik, Yashpal Singh
Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title_full Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title_fullStr Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title_full_unstemmed Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title_short Identification and In Silico Characterization of a Genetically Distinct Avian Rotavirus D Capsid Gene, VP7
title_sort identification and in silico characterization of a genetically distinct avian rotavirus d capsid gene, vp7
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027270/
https://www.ncbi.nlm.nih.gov/pubmed/29617342
http://dx.doi.org/10.3390/pathogens7020037
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