Cargando…

3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts

Fibroblast migration to damaged zones in different tissues is crucial to regenerate and recuperate their functional activity. However, fibroblast migration patterns have hardly been studied in disease terms. Here, we study this fundamental process in dermal and cardiac fibroblasts by means of microf...

Descripción completa

Detalles Bibliográficos
Autores principales: Pérez-Rodríguez, Sandra, Tomás-González, Esther, García-Aznar, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027294/
https://www.ncbi.nlm.nih.gov/pubmed/29895736
http://dx.doi.org/10.3390/bioengineering5020045
_version_ 1783336578390687744
author Pérez-Rodríguez, Sandra
Tomás-González, Esther
García-Aznar, José Manuel
author_facet Pérez-Rodríguez, Sandra
Tomás-González, Esther
García-Aznar, José Manuel
author_sort Pérez-Rodríguez, Sandra
collection PubMed
description Fibroblast migration to damaged zones in different tissues is crucial to regenerate and recuperate their functional activity. However, fibroblast migration patterns have hardly been studied in disease terms. Here, we study this fundamental process in dermal and cardiac fibroblasts by means of microfluidic-based experiments, which simulate a three-dimensional matrix in which fibroblasts are found in physiological conditions. Cardiac fibroblasts show a higher mean and effective speed, as well as greater contractile force, in comparison to dermal fibroblasts. In addition, we generate chemical gradients to study fibroblast response to platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β) gradients. Dermal fibroblasts were attracted to PDGF, whereas cardiac fibroblasts are not. Notwithstanding, cardiac fibroblasts increased their mean and effective velocity in the presence of TGF-β. Therefore, given that we observe that the application of these growth factors does not modify fibroblasts’ morphology, these alterations in the migration patterns may be due to an intracellular regulation.
format Online
Article
Text
id pubmed-6027294
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60272942018-07-13 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts Pérez-Rodríguez, Sandra Tomás-González, Esther García-Aznar, José Manuel Bioengineering (Basel) Article Fibroblast migration to damaged zones in different tissues is crucial to regenerate and recuperate their functional activity. However, fibroblast migration patterns have hardly been studied in disease terms. Here, we study this fundamental process in dermal and cardiac fibroblasts by means of microfluidic-based experiments, which simulate a three-dimensional matrix in which fibroblasts are found in physiological conditions. Cardiac fibroblasts show a higher mean and effective speed, as well as greater contractile force, in comparison to dermal fibroblasts. In addition, we generate chemical gradients to study fibroblast response to platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β) gradients. Dermal fibroblasts were attracted to PDGF, whereas cardiac fibroblasts are not. Notwithstanding, cardiac fibroblasts increased their mean and effective velocity in the presence of TGF-β. Therefore, given that we observe that the application of these growth factors does not modify fibroblasts’ morphology, these alterations in the migration patterns may be due to an intracellular regulation. MDPI 2018-06-12 /pmc/articles/PMC6027294/ /pubmed/29895736 http://dx.doi.org/10.3390/bioengineering5020045 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pérez-Rodríguez, Sandra
Tomás-González, Esther
García-Aznar, José Manuel
3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title_full 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title_fullStr 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title_full_unstemmed 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title_short 3D Cell Migration Studies for Chemotaxis on Microfluidic-Based Chips: A Comparison between Cardiac and Dermal Fibroblasts
title_sort 3d cell migration studies for chemotaxis on microfluidic-based chips: a comparison between cardiac and dermal fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027294/
https://www.ncbi.nlm.nih.gov/pubmed/29895736
http://dx.doi.org/10.3390/bioengineering5020045
work_keys_str_mv AT perezrodriguezsandra 3dcellmigrationstudiesforchemotaxisonmicrofluidicbasedchipsacomparisonbetweencardiacanddermalfibroblasts
AT tomasgonzalezesther 3dcellmigrationstudiesforchemotaxisonmicrofluidicbasedchipsacomparisonbetweencardiacanddermalfibroblasts
AT garciaaznarjosemanuel 3dcellmigrationstudiesforchemotaxisonmicrofluidicbasedchipsacomparisonbetweencardiacanddermalfibroblasts