Cargando…

Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells

Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-...

Descripción completa

Detalles Bibliográficos
Autores principales: Lensch, Michelle, Muise, Angela, White, Lisa, Badowski, Michael, Harris, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027308/
https://www.ncbi.nlm.nih.gov/pubmed/29757934
http://dx.doi.org/10.3390/biomedicines6020054
_version_ 1783336581688459264
author Lensch, Michelle
Muise, Angela
White, Lisa
Badowski, Michael
Harris, David
author_facet Lensch, Michelle
Muise, Angela
White, Lisa
Badowski, Michael
Harris, David
author_sort Lensch, Michelle
collection PubMed
description Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium.
format Online
Article
Text
id pubmed-6027308
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60273082018-07-13 Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells Lensch, Michelle Muise, Angela White, Lisa Badowski, Michael Harris, David Biomedicines Communication Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium. MDPI 2018-05-14 /pmc/articles/PMC6027308/ /pubmed/29757934 http://dx.doi.org/10.3390/biomedicines6020054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lensch, Michelle
Muise, Angela
White, Lisa
Badowski, Michael
Harris, David
Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title_full Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title_fullStr Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title_full_unstemmed Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title_short Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
title_sort comparison of synthetic media designed for expansion of adipose-derived mesenchymal stromal cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027308/
https://www.ncbi.nlm.nih.gov/pubmed/29757934
http://dx.doi.org/10.3390/biomedicines6020054
work_keys_str_mv AT lenschmichelle comparisonofsyntheticmediadesignedforexpansionofadiposederivedmesenchymalstromalcells
AT muiseangela comparisonofsyntheticmediadesignedforexpansionofadiposederivedmesenchymalstromalcells
AT whitelisa comparisonofsyntheticmediadesignedforexpansionofadiposederivedmesenchymalstromalcells
AT badowskimichael comparisonofsyntheticmediadesignedforexpansionofadiposederivedmesenchymalstromalcells
AT harrisdavid comparisonofsyntheticmediadesignedforexpansionofadiposederivedmesenchymalstromalcells