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Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells
Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027308/ https://www.ncbi.nlm.nih.gov/pubmed/29757934 http://dx.doi.org/10.3390/biomedicines6020054 |
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author | Lensch, Michelle Muise, Angela White, Lisa Badowski, Michael Harris, David |
author_facet | Lensch, Michelle Muise, Angela White, Lisa Badowski, Michael Harris, David |
author_sort | Lensch, Michelle |
collection | PubMed |
description | Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium. |
format | Online Article Text |
id | pubmed-6027308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60273082018-07-13 Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells Lensch, Michelle Muise, Angela White, Lisa Badowski, Michael Harris, David Biomedicines Communication Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium. MDPI 2018-05-14 /pmc/articles/PMC6027308/ /pubmed/29757934 http://dx.doi.org/10.3390/biomedicines6020054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Lensch, Michelle Muise, Angela White, Lisa Badowski, Michael Harris, David Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title | Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title_full | Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title_fullStr | Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title_full_unstemmed | Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title_short | Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells |
title_sort | comparison of synthetic media designed for expansion of adipose-derived mesenchymal stromal cells |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027308/ https://www.ncbi.nlm.nih.gov/pubmed/29757934 http://dx.doi.org/10.3390/biomedicines6020054 |
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