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Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells

Black phosphorus (BP) is a monolayer/multilayer two-dimensional (2D) nanomaterial, which has recently emerged as one of the most attractive 2D nanomaterials due to its fascinating physicochemical and optoelectronical properties. Layered BP may have promising applications in biomedical fields, such a...

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Autores principales: Song, Su-Jin, Shin, Yong Cheol, Lee, Hyun Uk, Kim, Bongju, Han, Dong-Wook, Lim, Dohyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027469/
https://www.ncbi.nlm.nih.gov/pubmed/29882820
http://dx.doi.org/10.3390/nano8060408
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author Song, Su-Jin
Shin, Yong Cheol
Lee, Hyun Uk
Kim, Bongju
Han, Dong-Wook
Lim, Dohyung
author_facet Song, Su-Jin
Shin, Yong Cheol
Lee, Hyun Uk
Kim, Bongju
Han, Dong-Wook
Lim, Dohyung
author_sort Song, Su-Jin
collection PubMed
description Black phosphorus (BP) is a monolayer/multilayer two-dimensional (2D) nanomaterial, which has recently emerged as one of the most attractive 2D nanomaterials due to its fascinating physicochemical and optoelectronical properties. Layered BP may have promising applications in biomedical fields, such as drug delivery, photodynamic/photothermal therapy and bioimaging, although its intrinsic toxicity has not been fully elucidated yet. In the present study, the cytotoxicological effects of layered BP on both cell metabolic activity and membrane integrity were investigated. Layered BPs were prepared using a modified ultrasonication-assisted solution method, and their physicochemical properties were characterized. The dose- and time-dependent cytotoxicity of layered BP was assessed against L-929 fibroblasts. Our findings indicate that the cytotoxicity of BPs is proportionally dependent on their concentration and exposure time, which is affected by the oxidative stress-mediated enzyme activity reduction and membrane disruption. On the other hand, layered BPs did not exhibit significant cytotoxicity at concentrations lower than 4 μg/mL. Therefore, it is suggested that layered BPs can be effectively utilized as therapeutic delivery carriers and imaging agents.
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spelling pubmed-60274692018-07-13 Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells Song, Su-Jin Shin, Yong Cheol Lee, Hyun Uk Kim, Bongju Han, Dong-Wook Lim, Dohyung Nanomaterials (Basel) Article Black phosphorus (BP) is a monolayer/multilayer two-dimensional (2D) nanomaterial, which has recently emerged as one of the most attractive 2D nanomaterials due to its fascinating physicochemical and optoelectronical properties. Layered BP may have promising applications in biomedical fields, such as drug delivery, photodynamic/photothermal therapy and bioimaging, although its intrinsic toxicity has not been fully elucidated yet. In the present study, the cytotoxicological effects of layered BP on both cell metabolic activity and membrane integrity were investigated. Layered BPs were prepared using a modified ultrasonication-assisted solution method, and their physicochemical properties were characterized. The dose- and time-dependent cytotoxicity of layered BP was assessed against L-929 fibroblasts. Our findings indicate that the cytotoxicity of BPs is proportionally dependent on their concentration and exposure time, which is affected by the oxidative stress-mediated enzyme activity reduction and membrane disruption. On the other hand, layered BPs did not exhibit significant cytotoxicity at concentrations lower than 4 μg/mL. Therefore, it is suggested that layered BPs can be effectively utilized as therapeutic delivery carriers and imaging agents. MDPI 2018-06-06 /pmc/articles/PMC6027469/ /pubmed/29882820 http://dx.doi.org/10.3390/nano8060408 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Su-Jin
Shin, Yong Cheol
Lee, Hyun Uk
Kim, Bongju
Han, Dong-Wook
Lim, Dohyung
Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title_full Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title_fullStr Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title_full_unstemmed Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title_short Dose- and Time-Dependent Cytotoxicity of Layered Black Phosphorus in Fibroblastic Cells
title_sort dose- and time-dependent cytotoxicity of layered black phosphorus in fibroblastic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027469/
https://www.ncbi.nlm.nih.gov/pubmed/29882820
http://dx.doi.org/10.3390/nano8060408
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