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Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design
Micro and small bioreactors are well described for use in bioprocess development in pre-production manufacture, using ultra-scale down and microfluidic methodology. However, the use of bioreactors to understand normal and pathophysiology by definition must be very different, and the constraints of t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027481/ https://www.ncbi.nlm.nih.gov/pubmed/29695077 http://dx.doi.org/10.3390/bioengineering5020032 |
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author | Selden, Clare Fuller, Barry |
author_facet | Selden, Clare Fuller, Barry |
author_sort | Selden, Clare |
collection | PubMed |
description | Micro and small bioreactors are well described for use in bioprocess development in pre-production manufacture, using ultra-scale down and microfluidic methodology. However, the use of bioreactors to understand normal and pathophysiology by definition must be very different, and the constraints of the physiological environment influence such bioreactor design. This review considers the key elements necessary to enable bioreactors to address three main areas associated with biological systems. All entail recreation of the in vivo cell niche as faithfully as possible, so that they may be used to study molecular and cellular changes in normal physiology, with a view to creating tissue-engineered grafts for clinical use; understanding the pathophysiology of disease at the molecular level; defining possible therapeutic targets; and enabling appropriate pharmaceutical testing on a truly representative organoid, thus enabling better drug design, and simultaneously creating the potential to reduce the numbers of animals in research. The premise explored is that not only cellular signalling cues, but also mechano-transduction from mechanical cues, play an important role. |
format | Online Article Text |
id | pubmed-6027481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60274812018-07-13 Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design Selden, Clare Fuller, Barry Bioengineering (Basel) Review Micro and small bioreactors are well described for use in bioprocess development in pre-production manufacture, using ultra-scale down and microfluidic methodology. However, the use of bioreactors to understand normal and pathophysiology by definition must be very different, and the constraints of the physiological environment influence such bioreactor design. This review considers the key elements necessary to enable bioreactors to address three main areas associated with biological systems. All entail recreation of the in vivo cell niche as faithfully as possible, so that they may be used to study molecular and cellular changes in normal physiology, with a view to creating tissue-engineered grafts for clinical use; understanding the pathophysiology of disease at the molecular level; defining possible therapeutic targets; and enabling appropriate pharmaceutical testing on a truly representative organoid, thus enabling better drug design, and simultaneously creating the potential to reduce the numbers of animals in research. The premise explored is that not only cellular signalling cues, but also mechano-transduction from mechanical cues, play an important role. MDPI 2018-04-24 /pmc/articles/PMC6027481/ /pubmed/29695077 http://dx.doi.org/10.3390/bioengineering5020032 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Selden, Clare Fuller, Barry Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title | Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title_full | Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title_fullStr | Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title_full_unstemmed | Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title_short | Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design |
title_sort | role of bioreactor technology in tissue engineering for clinical use and therapeutic target design |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027481/ https://www.ncbi.nlm.nih.gov/pubmed/29695077 http://dx.doi.org/10.3390/bioengineering5020032 |
work_keys_str_mv | AT seldenclare roleofbioreactortechnologyintissueengineeringforclinicaluseandtherapeutictargetdesign AT fullerbarry roleofbioreactortechnologyintissueengineeringforclinicaluseandtherapeutictargetdesign |