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Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, alt...

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Autores principales: Schimek, Katharina, Hsu, Hao-Hsiang, Boehme, Moritz, Kornet, Jacob Jan, Marx, Uwe, Lauster, Roland, Pörtner, Ralf, Lindner, Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027510/
https://www.ncbi.nlm.nih.gov/pubmed/29880746
http://dx.doi.org/10.3390/bioengineering5020043
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author Schimek, Katharina
Hsu, Hao-Hsiang
Boehme, Moritz
Kornet, Jacob Jan
Marx, Uwe
Lauster, Roland
Pörtner, Ralf
Lindner, Gerd
author_facet Schimek, Katharina
Hsu, Hao-Hsiang
Boehme, Moritz
Kornet, Jacob Jan
Marx, Uwe
Lauster, Roland
Pörtner, Ralf
Lindner, Gerd
author_sort Schimek, Katharina
collection PubMed
description The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.
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spelling pubmed-60275102018-07-13 Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications Schimek, Katharina Hsu, Hao-Hsiang Boehme, Moritz Kornet, Jacob Jan Marx, Uwe Lauster, Roland Pörtner, Ralf Lindner, Gerd Bioengineering (Basel) Article The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays. MDPI 2018-06-07 /pmc/articles/PMC6027510/ /pubmed/29880746 http://dx.doi.org/10.3390/bioengineering5020043 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schimek, Katharina
Hsu, Hao-Hsiang
Boehme, Moritz
Kornet, Jacob Jan
Marx, Uwe
Lauster, Roland
Pörtner, Ralf
Lindner, Gerd
Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title_full Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title_fullStr Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title_full_unstemmed Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title_short Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
title_sort bioengineering of a full-thickness skin equivalent in a 96-well insert format for substance permeation studies and organ-on-a-chip applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027510/
https://www.ncbi.nlm.nih.gov/pubmed/29880746
http://dx.doi.org/10.3390/bioengineering5020043
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