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Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease

BACKGROUND: Human Cytomegalovirus (HCMV) infection is problematic after kidney transplantation. Human mesangial cells along with human glomerular endothelial cells and podocytes constitute the renal glomerular vascular unit (GVU). HCMV infection of the GVU is poorly understood. METHODS: GVU cells in...

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Autores principales: Popik, Waldemar, Correa, Hernan, Khatua, Atanu, Aronoff, David M, Alcendor, Donald J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027753/
https://www.ncbi.nlm.nih.gov/pubmed/29977613
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author Popik, Waldemar
Correa, Hernan
Khatua, Atanu
Aronoff, David M
Alcendor, Donald J
author_facet Popik, Waldemar
Correa, Hernan
Khatua, Atanu
Aronoff, David M
Alcendor, Donald J
author_sort Popik, Waldemar
collection PubMed
description BACKGROUND: Human Cytomegalovirus (HCMV) infection is problematic after kidney transplantation. Human mesangial cells along with human glomerular endothelial cells and podocytes constitute the renal glomerular vascular unit (GVU). HCMV infection of the GVU is poorly understood. METHODS: GVU cells infectivity was analysed by microscopy and immunofluorescence. Cytokines profiles were measured by Luminex assays. Renal tissue analysis for HCMV infection was performed by immunohistochemistry. RESULTS: Mesangial cells and glomerular endothelial cells but not podocytes were permissive for both lab adapted and clinical strains of HCMV. Luminex analysis of cytokines expressed by mesangial cells exposed to the SBCMV clinical strain was examined. A Tricell infection model of the GVU maintains >90% viability with a unique cytokine profile. Finally, we show αSMA stained mesangial cells permissive for HCMV in renal tissue from a transplant patient. CONCLUSIONS: HCMV infection of mesangial cells induces angiogenic and proinflammatory cytokines that could contribute to glomerular inflammation.
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spelling pubmed-60277532019-02-01 Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease Popik, Waldemar Correa, Hernan Khatua, Atanu Aronoff, David M Alcendor, Donald J J Transl Sci Article BACKGROUND: Human Cytomegalovirus (HCMV) infection is problematic after kidney transplantation. Human mesangial cells along with human glomerular endothelial cells and podocytes constitute the renal glomerular vascular unit (GVU). HCMV infection of the GVU is poorly understood. METHODS: GVU cells infectivity was analysed by microscopy and immunofluorescence. Cytokines profiles were measured by Luminex assays. Renal tissue analysis for HCMV infection was performed by immunohistochemistry. RESULTS: Mesangial cells and glomerular endothelial cells but not podocytes were permissive for both lab adapted and clinical strains of HCMV. Luminex analysis of cytokines expressed by mesangial cells exposed to the SBCMV clinical strain was examined. A Tricell infection model of the GVU maintains >90% viability with a unique cytokine profile. Finally, we show αSMA stained mesangial cells permissive for HCMV in renal tissue from a transplant patient. CONCLUSIONS: HCMV infection of mesangial cells induces angiogenic and proinflammatory cytokines that could contribute to glomerular inflammation. 2018-05-24 2019-02 /pmc/articles/PMC6027753/ /pubmed/29977613 Text en This is an open-access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Popik, Waldemar
Correa, Hernan
Khatua, Atanu
Aronoff, David M
Alcendor, Donald J
Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title_full Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title_fullStr Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title_full_unstemmed Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title_short Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease
title_sort mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: implications for hcmv pathology in the glomerular vascular unit and post-transplant renal disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027753/
https://www.ncbi.nlm.nih.gov/pubmed/29977613
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