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Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70

Aspergillus flavus is a saprophytic fungus that infects corn, peanuts, tree nuts and other agriculturally important crops. Once the crop is infected the fungus has the potential to secrete one or more mycotoxins, the most carcinogenic of which is aflatoxin. Aflatoxin contaminated crops are deemed un...

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Autores principales: Gilbert, Matthew K., Mack, Brian M., Moore, Geromy G., Downey, Darlene L., Lebar, Matthew D., Joardar, Vinita, Losada, Liliana, Yu, JiuJiang, Nierman, William C., Bhatnagar, Deepak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028093/
https://www.ncbi.nlm.nih.gov/pubmed/29966003
http://dx.doi.org/10.1371/journal.pone.0199169
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author Gilbert, Matthew K.
Mack, Brian M.
Moore, Geromy G.
Downey, Darlene L.
Lebar, Matthew D.
Joardar, Vinita
Losada, Liliana
Yu, JiuJiang
Nierman, William C.
Bhatnagar, Deepak
author_facet Gilbert, Matthew K.
Mack, Brian M.
Moore, Geromy G.
Downey, Darlene L.
Lebar, Matthew D.
Joardar, Vinita
Losada, Liliana
Yu, JiuJiang
Nierman, William C.
Bhatnagar, Deepak
author_sort Gilbert, Matthew K.
collection PubMed
description Aspergillus flavus is a saprophytic fungus that infects corn, peanuts, tree nuts and other agriculturally important crops. Once the crop is infected the fungus has the potential to secrete one or more mycotoxins, the most carcinogenic of which is aflatoxin. Aflatoxin contaminated crops are deemed unfit for human or animal consumption, which results in both food and economic losses. Within A. flavus, two morphotypes exist: the S strains (small sclerotia) and L strains (large sclerotia). Significant morphological and physiological differences exist between the two morphotypes. For example, the S-morphotypes produces sclerotia that are smaller (< 400 μm), greater in quantity, and contain higher concentrations of aflatoxin than the L-morphotypes (>400 μm). The morphotypes also differ in pigmentation, pH homeostasis in culture and the number of spores produced. Here we report the first full genome sequence of an A. flavus S morphotype, strain AF70. We provide a comprehensive comparison of the A. flavus S-morphotype genome sequence with a previously sequenced genome of an L-morphotype strain (NRRL 3357), including an in-depth analysis of secondary metabolic clusters and the identification SNPs within their aflatoxin gene clusters.
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spelling pubmed-60280932018-07-19 Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70 Gilbert, Matthew K. Mack, Brian M. Moore, Geromy G. Downey, Darlene L. Lebar, Matthew D. Joardar, Vinita Losada, Liliana Yu, JiuJiang Nierman, William C. Bhatnagar, Deepak PLoS One Research Article Aspergillus flavus is a saprophytic fungus that infects corn, peanuts, tree nuts and other agriculturally important crops. Once the crop is infected the fungus has the potential to secrete one or more mycotoxins, the most carcinogenic of which is aflatoxin. Aflatoxin contaminated crops are deemed unfit for human or animal consumption, which results in both food and economic losses. Within A. flavus, two morphotypes exist: the S strains (small sclerotia) and L strains (large sclerotia). Significant morphological and physiological differences exist between the two morphotypes. For example, the S-morphotypes produces sclerotia that are smaller (< 400 μm), greater in quantity, and contain higher concentrations of aflatoxin than the L-morphotypes (>400 μm). The morphotypes also differ in pigmentation, pH homeostasis in culture and the number of spores produced. Here we report the first full genome sequence of an A. flavus S morphotype, strain AF70. We provide a comprehensive comparison of the A. flavus S-morphotype genome sequence with a previously sequenced genome of an L-morphotype strain (NRRL 3357), including an in-depth analysis of secondary metabolic clusters and the identification SNPs within their aflatoxin gene clusters. Public Library of Science 2018-07-02 /pmc/articles/PMC6028093/ /pubmed/29966003 http://dx.doi.org/10.1371/journal.pone.0199169 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Gilbert, Matthew K.
Mack, Brian M.
Moore, Geromy G.
Downey, Darlene L.
Lebar, Matthew D.
Joardar, Vinita
Losada, Liliana
Yu, JiuJiang
Nierman, William C.
Bhatnagar, Deepak
Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title_full Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title_fullStr Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title_full_unstemmed Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title_short Whole genome comparison of Aspergillus flavus L-morphotype strain NRRL 3357 (type) and S-morphotype strain AF70
title_sort whole genome comparison of aspergillus flavus l-morphotype strain nrrl 3357 (type) and s-morphotype strain af70
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028093/
https://www.ncbi.nlm.nih.gov/pubmed/29966003
http://dx.doi.org/10.1371/journal.pone.0199169
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