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Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis

Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Braz...

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Autores principales: Reymão, Tammy Kathlyn Amaral, Fumian, Tulio Machado, Justino, Maria Cleonice Aguiar, Hernandez, Juliana Merces, Bandeira, Renato Silva, Lucena, Maria Silvia Sousa, Teixeira, Dielle Monteiro, Farias, Fredison Pinheiro, Silva, Luciana Damascena, Linhares, Alexandre Costa, Gabbay, Yvone Benchimol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028094/
https://www.ncbi.nlm.nih.gov/pubmed/29965979
http://dx.doi.org/10.1371/journal.pone.0199763
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author Reymão, Tammy Kathlyn Amaral
Fumian, Tulio Machado
Justino, Maria Cleonice Aguiar
Hernandez, Juliana Merces
Bandeira, Renato Silva
Lucena, Maria Silvia Sousa
Teixeira, Dielle Monteiro
Farias, Fredison Pinheiro
Silva, Luciana Damascena
Linhares, Alexandre Costa
Gabbay, Yvone Benchimol
author_facet Reymão, Tammy Kathlyn Amaral
Fumian, Tulio Machado
Justino, Maria Cleonice Aguiar
Hernandez, Juliana Merces
Bandeira, Renato Silva
Lucena, Maria Silvia Sousa
Teixeira, Dielle Monteiro
Farias, Fredison Pinheiro
Silva, Luciana Damascena
Linhares, Alexandre Costa
Gabbay, Yvone Benchimol
author_sort Reymão, Tammy Kathlyn Amaral
collection PubMed
description Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Brazil, and also correlated viral RNA levels in their blood and stool with clinical severity. For this purpose, paired stool and serum samples were collected from 445 pediatric patients, ≤9 years between March 2012 and June 2015. Enzyme-linked immunosorbent assay (EIA) was used to detect NoV in stool and reverse transcription quantitative PCR (RT-qPCR) used to quantify NoV RNA levels in sera (RNAemia) and in the positive stool. Positives samples were characterized by the partial ORF1/2 region sequence of viral genome. NoV antigen was detected in 24.3% (108/445) of stool samples, with RNAemia also present in 20.4% (22/108). RNAemia and a high stool viral load (>10(7) genome copies/gram of faeces) were associated with longer hospitalizations. The prevalent genotypes were GII.4 Sydney_2012 (71.6%-58/81) and New Orleans_2009 (6.2%-5/81) variants. Eight other genotypes belonging to GII were detected and four of them were recombinant strains. All sera were characterized as GII.4 and shared 100% similarity with their stool. The results suggest that the dissemination of NoV to the blood stream is not uncommon and may be related to increased faecal viral loads and disease severity.
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spelling pubmed-60280942018-07-19 Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis Reymão, Tammy Kathlyn Amaral Fumian, Tulio Machado Justino, Maria Cleonice Aguiar Hernandez, Juliana Merces Bandeira, Renato Silva Lucena, Maria Silvia Sousa Teixeira, Dielle Monteiro Farias, Fredison Pinheiro Silva, Luciana Damascena Linhares, Alexandre Costa Gabbay, Yvone Benchimol PLoS One Research Article Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Brazil, and also correlated viral RNA levels in their blood and stool with clinical severity. For this purpose, paired stool and serum samples were collected from 445 pediatric patients, ≤9 years between March 2012 and June 2015. Enzyme-linked immunosorbent assay (EIA) was used to detect NoV in stool and reverse transcription quantitative PCR (RT-qPCR) used to quantify NoV RNA levels in sera (RNAemia) and in the positive stool. Positives samples were characterized by the partial ORF1/2 region sequence of viral genome. NoV antigen was detected in 24.3% (108/445) of stool samples, with RNAemia also present in 20.4% (22/108). RNAemia and a high stool viral load (>10(7) genome copies/gram of faeces) were associated with longer hospitalizations. The prevalent genotypes were GII.4 Sydney_2012 (71.6%-58/81) and New Orleans_2009 (6.2%-5/81) variants. Eight other genotypes belonging to GII were detected and four of them were recombinant strains. All sera were characterized as GII.4 and shared 100% similarity with their stool. The results suggest that the dissemination of NoV to the blood stream is not uncommon and may be related to increased faecal viral loads and disease severity. Public Library of Science 2018-07-02 /pmc/articles/PMC6028094/ /pubmed/29965979 http://dx.doi.org/10.1371/journal.pone.0199763 Text en © 2018 Reymão et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Reymão, Tammy Kathlyn Amaral
Fumian, Tulio Machado
Justino, Maria Cleonice Aguiar
Hernandez, Juliana Merces
Bandeira, Renato Silva
Lucena, Maria Silvia Sousa
Teixeira, Dielle Monteiro
Farias, Fredison Pinheiro
Silva, Luciana Damascena
Linhares, Alexandre Costa
Gabbay, Yvone Benchimol
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title_full Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title_fullStr Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title_full_unstemmed Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title_short Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis
title_sort norovirus rna in serum associated with increased fecal viral load in children: detection, quantification and molecular analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028094/
https://www.ncbi.nlm.nih.gov/pubmed/29965979
http://dx.doi.org/10.1371/journal.pone.0199763
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