Ultraviolet Hyperspectral Interferometric Microscopy

Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to ov...

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Autores principales: Ojaghi, Ashkan, Fay, Meredith E., Lam, Wilbur A., Robles, Francisco E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028425/
https://www.ncbi.nlm.nih.gov/pubmed/29967322
http://dx.doi.org/10.1038/s41598-018-28208-0
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author Ojaghi, Ashkan
Fay, Meredith E.
Lam, Wilbur A.
Robles, Francisco E.
author_facet Ojaghi, Ashkan
Fay, Meredith E.
Lam, Wilbur A.
Robles, Francisco E.
author_sort Ojaghi, Ashkan
collection PubMed
description Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells.
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spelling pubmed-60284252018-07-09 Ultraviolet Hyperspectral Interferometric Microscopy Ojaghi, Ashkan Fay, Meredith E. Lam, Wilbur A. Robles, Francisco E. Sci Rep Article Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells. Nature Publishing Group UK 2018-07-02 /pmc/articles/PMC6028425/ /pubmed/29967322 http://dx.doi.org/10.1038/s41598-018-28208-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ojaghi, Ashkan
Fay, Meredith E.
Lam, Wilbur A.
Robles, Francisco E.
Ultraviolet Hyperspectral Interferometric Microscopy
title Ultraviolet Hyperspectral Interferometric Microscopy
title_full Ultraviolet Hyperspectral Interferometric Microscopy
title_fullStr Ultraviolet Hyperspectral Interferometric Microscopy
title_full_unstemmed Ultraviolet Hyperspectral Interferometric Microscopy
title_short Ultraviolet Hyperspectral Interferometric Microscopy
title_sort ultraviolet hyperspectral interferometric microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028425/
https://www.ncbi.nlm.nih.gov/pubmed/29967322
http://dx.doi.org/10.1038/s41598-018-28208-0
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