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Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028518/ https://www.ncbi.nlm.nih.gov/pubmed/29899037 http://dx.doi.org/10.1084/jem.20171384 |
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author | Jensen, Christina T. Åhsberg, Josefine Sommarin, Mikael N.E. Strid, Tobias Somasundaram, Rajesh Okuyama, Kazuki Ungerbäck, Jonas Kupari, Jussi Airaksinen, Matti S. Lang, Stefan Bryder, David Soneji, Shamit Karlsson, Göran Sigvardsson, Mikael |
author_facet | Jensen, Christina T. Åhsberg, Josefine Sommarin, Mikael N.E. Strid, Tobias Somasundaram, Rajesh Okuyama, Kazuki Ungerbäck, Jonas Kupari, Jussi Airaksinen, Matti S. Lang, Stefan Bryder, David Soneji, Shamit Karlsson, Göran Sigvardsson, Mikael |
author_sort | Jensen, Christina T. |
collection | PubMed |
description | To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19(+) progenitor compartment. |
format | Online Article Text |
id | pubmed-6028518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60285182019-01-02 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis Jensen, Christina T. Åhsberg, Josefine Sommarin, Mikael N.E. Strid, Tobias Somasundaram, Rajesh Okuyama, Kazuki Ungerbäck, Jonas Kupari, Jussi Airaksinen, Matti S. Lang, Stefan Bryder, David Soneji, Shamit Karlsson, Göran Sigvardsson, Mikael J Exp Med Research Articles To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19(+) progenitor compartment. Rockefeller University Press 2018-07-02 /pmc/articles/PMC6028518/ /pubmed/29899037 http://dx.doi.org/10.1084/jem.20171384 Text en © 2018 Jensen et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Jensen, Christina T. Åhsberg, Josefine Sommarin, Mikael N.E. Strid, Tobias Somasundaram, Rajesh Okuyama, Kazuki Ungerbäck, Jonas Kupari, Jussi Airaksinen, Matti S. Lang, Stefan Bryder, David Soneji, Shamit Karlsson, Göran Sigvardsson, Mikael Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title | Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title_full | Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title_fullStr | Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title_full_unstemmed | Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title_short | Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis |
title_sort | dissection of progenitor compartments resolves developmental trajectories in b-lymphopoiesis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028518/ https://www.ncbi.nlm.nih.gov/pubmed/29899037 http://dx.doi.org/10.1084/jem.20171384 |
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