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The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion
Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharo...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028538/ https://www.ncbi.nlm.nih.gov/pubmed/29875260 http://dx.doi.org/10.1083/jcb.201709009 |
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author | Sing, Tina L. Hung, Minnie P. Ohnuki, Shinsuke Suzuki, Godai San Luis, Bryan-Joseph McClain, Melainia Unruh, Jay R. Yu, Zulin Ou, Jiongwen Marshall-Sheppard, Jesse Huh, Won-Ki Costanzo, Michael Boone, Charles Ohya, Yoshikazu Jaspersen, Sue L. Brown, Grant W. |
author_facet | Sing, Tina L. Hung, Minnie P. Ohnuki, Shinsuke Suzuki, Godai San Luis, Bryan-Joseph McClain, Melainia Unruh, Jay R. Yu, Zulin Ou, Jiongwen Marshall-Sheppard, Jesse Huh, Won-Ki Costanzo, Michael Boone, Charles Ohya, Yoshikazu Jaspersen, Sue L. Brown, Grant W. |
author_sort | Sing, Tina L. |
collection | PubMed |
description | Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae “remodels the structure of chromatin” (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance. |
format | Online Article Text |
id | pubmed-6028538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60285382019-01-02 The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion Sing, Tina L. Hung, Minnie P. Ohnuki, Shinsuke Suzuki, Godai San Luis, Bryan-Joseph McClain, Melainia Unruh, Jay R. Yu, Zulin Ou, Jiongwen Marshall-Sheppard, Jesse Huh, Won-Ki Costanzo, Michael Boone, Charles Ohya, Yoshikazu Jaspersen, Sue L. Brown, Grant W. J Cell Biol Research Articles Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae “remodels the structure of chromatin” (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance. Rockefeller University Press 2018-07-02 /pmc/articles/PMC6028538/ /pubmed/29875260 http://dx.doi.org/10.1083/jcb.201709009 Text en © 2018 Sing et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Sing, Tina L. Hung, Minnie P. Ohnuki, Shinsuke Suzuki, Godai San Luis, Bryan-Joseph McClain, Melainia Unruh, Jay R. Yu, Zulin Ou, Jiongwen Marshall-Sheppard, Jesse Huh, Won-Ki Costanzo, Michael Boone, Charles Ohya, Yoshikazu Jaspersen, Sue L. Brown, Grant W. The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title | The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title_full | The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title_fullStr | The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title_full_unstemmed | The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title_short | The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion |
title_sort | budding yeast rsc complex maintains ploidy by promoting spindle pole body insertion |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028538/ https://www.ncbi.nlm.nih.gov/pubmed/29875260 http://dx.doi.org/10.1083/jcb.201709009 |
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