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HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion
Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028553/ https://www.ncbi.nlm.nih.gov/pubmed/29891722 http://dx.doi.org/10.1083/jcb.201710051 |
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author | MacDonald, Ewan Brown, Louise Selvais, Arnaud Liu, Han Waring, Thomas Newman, Daniel Bithell, Jessica Grimes, Douglas Urbé, Sylvie Clague, Michael J. Zech, Tobias |
author_facet | MacDonald, Ewan Brown, Louise Selvais, Arnaud Liu, Han Waring, Thomas Newman, Daniel Bithell, Jessica Grimes, Douglas Urbé, Sylvie Clague, Michael J. Zech, Tobias |
author_sort | MacDonald, Ewan |
collection | PubMed |
description | Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor–regulated tyrosine kinase substrate (HRS) in promoting the constitutive recycling of transmembrane proteins. We find that endosomal localization of the actin nucleating factor Wiscott-Aldrich syndrome protein and SCAR homologue (WASH) requires HRS, which occupies adjacent endosomal subdomains. Depletion of HRS results in defective constitutive recycling of epidermal growth factor receptor and the matrix metalloproteinase MT1–MMP, leading to their accumulation in internal compartments. We show that direct interactions with endosomal actin are required for efficient recycling and use a model system of chimeric transferrin receptor trafficking to show that an actin-binding motif can counteract an ubiquitin signal for lysosomal sorting. Directed receptor recycling is used by cancer cells to achieve invasive migration. Accordingly, abrogating HRS- and actin-dependent MT1-MMP recycling results in defective matrix degradation and invasion of triple-negative breast cancer cells. |
format | Online Article Text |
id | pubmed-6028553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60285532018-07-05 HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion MacDonald, Ewan Brown, Louise Selvais, Arnaud Liu, Han Waring, Thomas Newman, Daniel Bithell, Jessica Grimes, Douglas Urbé, Sylvie Clague, Michael J. Zech, Tobias J Cell Biol Research Articles Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor–regulated tyrosine kinase substrate (HRS) in promoting the constitutive recycling of transmembrane proteins. We find that endosomal localization of the actin nucleating factor Wiscott-Aldrich syndrome protein and SCAR homologue (WASH) requires HRS, which occupies adjacent endosomal subdomains. Depletion of HRS results in defective constitutive recycling of epidermal growth factor receptor and the matrix metalloproteinase MT1–MMP, leading to their accumulation in internal compartments. We show that direct interactions with endosomal actin are required for efficient recycling and use a model system of chimeric transferrin receptor trafficking to show that an actin-binding motif can counteract an ubiquitin signal for lysosomal sorting. Directed receptor recycling is used by cancer cells to achieve invasive migration. Accordingly, abrogating HRS- and actin-dependent MT1-MMP recycling results in defective matrix degradation and invasion of triple-negative breast cancer cells. Rockefeller University Press 2018-07-02 /pmc/articles/PMC6028553/ /pubmed/29891722 http://dx.doi.org/10.1083/jcb.201710051 Text en © 2018 MacDonald et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles MacDonald, Ewan Brown, Louise Selvais, Arnaud Liu, Han Waring, Thomas Newman, Daniel Bithell, Jessica Grimes, Douglas Urbé, Sylvie Clague, Michael J. Zech, Tobias HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title | HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title_full | HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title_fullStr | HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title_full_unstemmed | HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title_short | HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion |
title_sort | hrs–wash axis governs actin-mediated endosomal recycling and cell invasion |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028553/ https://www.ncbi.nlm.nih.gov/pubmed/29891722 http://dx.doi.org/10.1083/jcb.201710051 |
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