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An ultra-fast mechanically active cell culture substrate

We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell m...

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Autores principales: Poulin, Alexandre, Imboden, Matthias, Sorba, Francesca, Grazioli, Serge, Martin-Olmos, Cristina, Rosset, Samuel, Shea, Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028580/
https://www.ncbi.nlm.nih.gov/pubmed/29967520
http://dx.doi.org/10.1038/s41598-018-27915-y
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author Poulin, Alexandre
Imboden, Matthias
Sorba, Francesca
Grazioli, Serge
Martin-Olmos, Cristina
Rosset, Samuel
Shea, Herbert
author_facet Poulin, Alexandre
Imboden, Matthias
Sorba, Francesca
Grazioli, Serge
Martin-Olmos, Cristina
Rosset, Samuel
Shea, Herbert
author_sort Poulin, Alexandre
collection PubMed
description We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell monolayer is cultured on a dielectric elastomer actuator (DEA) made of a 30 μm thick silicone membrane sandwiched between stretchable electrodes. A potential difference of several kV’s is applied across the electrodes to generate electrostatic forces and induce mechanical deformation of the silicone membrane. The DEA cell stretcher we present here applies up to 38% tensile and 12% compressive strain, while allowing real-time live cell imaging. It reaches the set strain in well under 1 ms and generates strain rates as high as 870 s(−1), or 87%/ms. With the unique capability to stretch and compress cells, our ultra-fast device can reproduce the rich mechanical environment experienced by cells in normal physiological conditions, as well as in extreme conditions such as blunt force trauma. This new tool will help solving lingering questions in the field of mechanobiology, including the strain-rate dependence of axonal injury and the role of mechanics in actin stress fiber kinetics.
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spelling pubmed-60285802018-07-09 An ultra-fast mechanically active cell culture substrate Poulin, Alexandre Imboden, Matthias Sorba, Francesca Grazioli, Serge Martin-Olmos, Cristina Rosset, Samuel Shea, Herbert Sci Rep Article We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell monolayer is cultured on a dielectric elastomer actuator (DEA) made of a 30 μm thick silicone membrane sandwiched between stretchable electrodes. A potential difference of several kV’s is applied across the electrodes to generate electrostatic forces and induce mechanical deformation of the silicone membrane. The DEA cell stretcher we present here applies up to 38% tensile and 12% compressive strain, while allowing real-time live cell imaging. It reaches the set strain in well under 1 ms and generates strain rates as high as 870 s(−1), or 87%/ms. With the unique capability to stretch and compress cells, our ultra-fast device can reproduce the rich mechanical environment experienced by cells in normal physiological conditions, as well as in extreme conditions such as blunt force trauma. This new tool will help solving lingering questions in the field of mechanobiology, including the strain-rate dependence of axonal injury and the role of mechanics in actin stress fiber kinetics. Nature Publishing Group UK 2018-07-02 /pmc/articles/PMC6028580/ /pubmed/29967520 http://dx.doi.org/10.1038/s41598-018-27915-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Poulin, Alexandre
Imboden, Matthias
Sorba, Francesca
Grazioli, Serge
Martin-Olmos, Cristina
Rosset, Samuel
Shea, Herbert
An ultra-fast mechanically active cell culture substrate
title An ultra-fast mechanically active cell culture substrate
title_full An ultra-fast mechanically active cell culture substrate
title_fullStr An ultra-fast mechanically active cell culture substrate
title_full_unstemmed An ultra-fast mechanically active cell culture substrate
title_short An ultra-fast mechanically active cell culture substrate
title_sort ultra-fast mechanically active cell culture substrate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028580/
https://www.ncbi.nlm.nih.gov/pubmed/29967520
http://dx.doi.org/10.1038/s41598-018-27915-y
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