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Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population

Genetic divergence for nitrogen utilization in germplasms is important in wheat breeding programs, especially for low nitrogen input management. In this study, a nested association mapping (NAM) population, derived from “Yanzhan 1” (a Chinese domesticated cultivar) crossed with “Hussar” (a British d...

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Autores principales: Ren, Deqiang, Fang, Xiaojian, Jiang, Peng, Zhang, Guangxu, Hu, Junmei, Wang, Xiaoqian, Meng, Qing, Cui, Weian, Lan, Shengjie, Ma, Xin, Wang, Hongwei, Kong, Lingrang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028695/
https://www.ncbi.nlm.nih.gov/pubmed/29997636
http://dx.doi.org/10.3389/fpls.2018.00845
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author Ren, Deqiang
Fang, Xiaojian
Jiang, Peng
Zhang, Guangxu
Hu, Junmei
Wang, Xiaoqian
Meng, Qing
Cui, Weian
Lan, Shengjie
Ma, Xin
Wang, Hongwei
Kong, Lingrang
author_facet Ren, Deqiang
Fang, Xiaojian
Jiang, Peng
Zhang, Guangxu
Hu, Junmei
Wang, Xiaoqian
Meng, Qing
Cui, Weian
Lan, Shengjie
Ma, Xin
Wang, Hongwei
Kong, Lingrang
author_sort Ren, Deqiang
collection PubMed
description Genetic divergence for nitrogen utilization in germplasms is important in wheat breeding programs, especially for low nitrogen input management. In this study, a nested association mapping (NAM) population, derived from “Yanzhan 1” (a Chinese domesticated cultivar) crossed with “Hussar” (a British domesticated cultivar) and another three semi-wild wheat varieties, namely, “Cayazheda 29” (Triticum aestivum ssp. tibetanum Shao), “Yunnan” (T. aestivum ssp. yunnanense King), and “Yutian” (T. aestivum petropavloski Udats et Migusch), was used to detect quantitative trait loci (QTLs) for nitrogen utilization at the seedling stage. An integrated genetic map was constructed using 2,059 single nucleotide polymorphism (SNP) markers from a 90 K SNP chip, with a total coverage of 2,355.75 cM and an average marker spacing of 1.13 cM. A total of 67 QTLs for RDW (root dry weight), SDW (shoot dry weight), TDW (total dry weight), and RSDW (root to shoot ratio) were identified under normal nitrogen conditions (N(+)) and nitrogen deficient conditions (N(−)). Twenty-three of these QTLs were only detected under N(−) conditions. Moreover, 23 favorable QTLs were identified in the domesticated cultivar Yanzhan 1, 15 of which were detected under N(+) conditions, while only four were detected under N(−) conditions. In contrast, the semi-wild cultivars contributed more favorable N(−)−specific QTLs (eight from Cayazheda 29; nine from Yunnan), which could be further explored for breeding cultivars adapted to nitrogen-deficient conditions. In particular, QRSDW-5A.1 from YN should be further evaluated using high-resolution mapping.
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spelling pubmed-60286952018-07-11 Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population Ren, Deqiang Fang, Xiaojian Jiang, Peng Zhang, Guangxu Hu, Junmei Wang, Xiaoqian Meng, Qing Cui, Weian Lan, Shengjie Ma, Xin Wang, Hongwei Kong, Lingrang Front Plant Sci Plant Science Genetic divergence for nitrogen utilization in germplasms is important in wheat breeding programs, especially for low nitrogen input management. In this study, a nested association mapping (NAM) population, derived from “Yanzhan 1” (a Chinese domesticated cultivar) crossed with “Hussar” (a British domesticated cultivar) and another three semi-wild wheat varieties, namely, “Cayazheda 29” (Triticum aestivum ssp. tibetanum Shao), “Yunnan” (T. aestivum ssp. yunnanense King), and “Yutian” (T. aestivum petropavloski Udats et Migusch), was used to detect quantitative trait loci (QTLs) for nitrogen utilization at the seedling stage. An integrated genetic map was constructed using 2,059 single nucleotide polymorphism (SNP) markers from a 90 K SNP chip, with a total coverage of 2,355.75 cM and an average marker spacing of 1.13 cM. A total of 67 QTLs for RDW (root dry weight), SDW (shoot dry weight), TDW (total dry weight), and RSDW (root to shoot ratio) were identified under normal nitrogen conditions (N(+)) and nitrogen deficient conditions (N(−)). Twenty-three of these QTLs were only detected under N(−) conditions. Moreover, 23 favorable QTLs were identified in the domesticated cultivar Yanzhan 1, 15 of which were detected under N(+) conditions, while only four were detected under N(−) conditions. In contrast, the semi-wild cultivars contributed more favorable N(−)−specific QTLs (eight from Cayazheda 29; nine from Yunnan), which could be further explored for breeding cultivars adapted to nitrogen-deficient conditions. In particular, QRSDW-5A.1 from YN should be further evaluated using high-resolution mapping. Frontiers Media S.A. 2018-06-26 /pmc/articles/PMC6028695/ /pubmed/29997636 http://dx.doi.org/10.3389/fpls.2018.00845 Text en Copyright © 2018 Ren, Fang, Jiang, Zhang, Hu, Wang, Meng, Cui, Lan, Ma, Wang and Kong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ren, Deqiang
Fang, Xiaojian
Jiang, Peng
Zhang, Guangxu
Hu, Junmei
Wang, Xiaoqian
Meng, Qing
Cui, Weian
Lan, Shengjie
Ma, Xin
Wang, Hongwei
Kong, Lingrang
Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title_full Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title_fullStr Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title_full_unstemmed Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title_short Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population
title_sort genetic architecture of nitrogen-deficiency tolerance in wheat seedlings based on a nested association mapping (nam) population
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028695/
https://www.ncbi.nlm.nih.gov/pubmed/29997636
http://dx.doi.org/10.3389/fpls.2018.00845
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