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GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis

Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally c...

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Autores principales: Xu, Zhaolong, Raza, Qasim, Xu, Ling, He, Xiaolan, Huang, Yihong, Yi, Jinxin, Zhang, Dayong, Shao, Hong-Bo, Ma, Hongxiang, Ali, Zulfiqar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028721/
https://www.ncbi.nlm.nih.gov/pubmed/29997634
http://dx.doi.org/10.3389/fpls.2018.00809
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author Xu, Zhaolong
Raza, Qasim
Xu, Ling
He, Xiaolan
Huang, Yihong
Yi, Jinxin
Zhang, Dayong
Shao, Hong-Bo
Ma, Hongxiang
Ali, Zulfiqar
author_facet Xu, Zhaolong
Raza, Qasim
Xu, Ling
He, Xiaolan
Huang, Yihong
Yi, Jinxin
Zhang, Dayong
Shao, Hong-Bo
Ma, Hongxiang
Ali, Zulfiqar
author_sort Xu, Zhaolong
collection PubMed
description Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C(2)H(2)-type zinc-finger structure. The normalized expression (log(2) ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement.
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spelling pubmed-60287212018-07-11 GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis Xu, Zhaolong Raza, Qasim Xu, Ling He, Xiaolan Huang, Yihong Yi, Jinxin Zhang, Dayong Shao, Hong-Bo Ma, Hongxiang Ali, Zulfiqar Front Plant Sci Plant Science Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C(2)H(2)-type zinc-finger structure. The normalized expression (log(2) ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. Frontiers Media S.A. 2018-06-26 /pmc/articles/PMC6028721/ /pubmed/29997634 http://dx.doi.org/10.3389/fpls.2018.00809 Text en Copyright © 2018 Xu, Raza, Xu, He, Huang, Yi, Zhang, Shao, Ma and Ali. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Zhaolong
Raza, Qasim
Xu, Ling
He, Xiaolan
Huang, Yihong
Yi, Jinxin
Zhang, Dayong
Shao, Hong-Bo
Ma, Hongxiang
Ali, Zulfiqar
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title_full GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title_fullStr GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title_full_unstemmed GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title_short GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
title_sort gmwrky49, a salt-responsive nuclear protein, improved root length and governed better salinity tolerance in transgenic arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028721/
https://www.ncbi.nlm.nih.gov/pubmed/29997634
http://dx.doi.org/10.3389/fpls.2018.00809
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