Cargando…
Microbial and host immune factors as drivers of COPD
Compartmentalisation of the respiratory tract microbiota in patients with different chronic obstructive pulmonary disease (COPD) severity degrees needs to be systematically investigated. In addition, it is unknown if the inflammatory and emphysematous milieux in patients with COPD are associated wit...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Respiratory Society
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028745/ https://www.ncbi.nlm.nih.gov/pubmed/29992131 http://dx.doi.org/10.1183/23120541.00015-2018 |
_version_ | 1783336832698679296 |
---|---|
author | Mika, Moana Nita, Izabela Morf, Laura Qi, Weihong Beyeler, Seraina Bernasconi, Eric Marsland, Benjamin J. Ott, Sebastian R. von Garnier, Christophe Hilty, Markus |
author_facet | Mika, Moana Nita, Izabela Morf, Laura Qi, Weihong Beyeler, Seraina Bernasconi, Eric Marsland, Benjamin J. Ott, Sebastian R. von Garnier, Christophe Hilty, Markus |
author_sort | Mika, Moana |
collection | PubMed |
description | Compartmentalisation of the respiratory tract microbiota in patients with different chronic obstructive pulmonary disease (COPD) severity degrees needs to be systematically investigated. In addition, it is unknown if the inflammatory and emphysematous milieux in patients with COPD are associated with changes in the respiratory tract microbiota and host macrophage gene expression. We performed a cross-sectional study to compare non-COPD controls (n=10) to COPD patients (n=32) with different disease severity degrees. Samples (n=187) were obtained from different sites of the upper and lower respiratory tract. Microbiota analyses were performed by 16S ribosomal RNA gene sequencing and host gene expression analyses by quantitative real-time PCR of distinct markers of bronchoalveolar lavage cells. Overall, the microbial communities of severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) grade 3/4) patients clustered significantly differently to controls and less severe COPD (GOLD 1/2) patients (permutational multivariate ANOVA (MANOVA), p=0.001). However, we could not detect significant associations between the different sampling sites in the lower airways. In addition, the chosen set of host gene expression markers significantly separated COPD GOLD 3/4 patients, and we found correlations between the composition of the microbiota and the host data. In conclusion, this study demonstrates associations between host gene expression and microbiota profiles that may influence the course of COPD. |
format | Online Article Text |
id | pubmed-6028745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60287452018-07-10 Microbial and host immune factors as drivers of COPD Mika, Moana Nita, Izabela Morf, Laura Qi, Weihong Beyeler, Seraina Bernasconi, Eric Marsland, Benjamin J. Ott, Sebastian R. von Garnier, Christophe Hilty, Markus ERJ Open Res Original Article Compartmentalisation of the respiratory tract microbiota in patients with different chronic obstructive pulmonary disease (COPD) severity degrees needs to be systematically investigated. In addition, it is unknown if the inflammatory and emphysematous milieux in patients with COPD are associated with changes in the respiratory tract microbiota and host macrophage gene expression. We performed a cross-sectional study to compare non-COPD controls (n=10) to COPD patients (n=32) with different disease severity degrees. Samples (n=187) were obtained from different sites of the upper and lower respiratory tract. Microbiota analyses were performed by 16S ribosomal RNA gene sequencing and host gene expression analyses by quantitative real-time PCR of distinct markers of bronchoalveolar lavage cells. Overall, the microbial communities of severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) grade 3/4) patients clustered significantly differently to controls and less severe COPD (GOLD 1/2) patients (permutational multivariate ANOVA (MANOVA), p=0.001). However, we could not detect significant associations between the different sampling sites in the lower airways. In addition, the chosen set of host gene expression markers significantly separated COPD GOLD 3/4 patients, and we found correlations between the composition of the microbiota and the host data. In conclusion, this study demonstrates associations between host gene expression and microbiota profiles that may influence the course of COPD. European Respiratory Society 2018-07-03 /pmc/articles/PMC6028745/ /pubmed/29992131 http://dx.doi.org/10.1183/23120541.00015-2018 Text en Copyright ©ERS 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. |
spellingShingle | Original Article Mika, Moana Nita, Izabela Morf, Laura Qi, Weihong Beyeler, Seraina Bernasconi, Eric Marsland, Benjamin J. Ott, Sebastian R. von Garnier, Christophe Hilty, Markus Microbial and host immune factors as drivers of COPD |
title | Microbial and host immune factors as drivers of COPD |
title_full | Microbial and host immune factors as drivers of COPD |
title_fullStr | Microbial and host immune factors as drivers of COPD |
title_full_unstemmed | Microbial and host immune factors as drivers of COPD |
title_short | Microbial and host immune factors as drivers of COPD |
title_sort | microbial and host immune factors as drivers of copd |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028745/ https://www.ncbi.nlm.nih.gov/pubmed/29992131 http://dx.doi.org/10.1183/23120541.00015-2018 |
work_keys_str_mv | AT mikamoana microbialandhostimmunefactorsasdriversofcopd AT nitaizabela microbialandhostimmunefactorsasdriversofcopd AT morflaura microbialandhostimmunefactorsasdriversofcopd AT qiweihong microbialandhostimmunefactorsasdriversofcopd AT beyelerseraina microbialandhostimmunefactorsasdriversofcopd AT bernasconieric microbialandhostimmunefactorsasdriversofcopd AT marslandbenjaminj microbialandhostimmunefactorsasdriversofcopd AT ottsebastianr microbialandhostimmunefactorsasdriversofcopd AT vongarnierchristophe microbialandhostimmunefactorsasdriversofcopd AT hiltymarkus microbialandhostimmunefactorsasdriversofcopd |