Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells

Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing c...

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Autores principales: Szczepanski, Artur, Owczarek, Katarzyna, Milewska, Aleksandra, Baster, Zbigniew, Rajfur, Zenon, Mitchell, Judy A., Pyrc, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029178/
https://www.ncbi.nlm.nih.gov/pubmed/29970183
http://dx.doi.org/10.1186/s13567-018-0551-9
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author Szczepanski, Artur
Owczarek, Katarzyna
Milewska, Aleksandra
Baster, Zbigniew
Rajfur, Zenon
Mitchell, Judy A.
Pyrc, Krzysztof
author_facet Szczepanski, Artur
Owczarek, Katarzyna
Milewska, Aleksandra
Baster, Zbigniew
Rajfur, Zenon
Mitchell, Judy A.
Pyrc, Krzysztof
author_sort Szczepanski, Artur
collection PubMed
description Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0551-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-60291782018-07-09 Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells Szczepanski, Artur Owczarek, Katarzyna Milewska, Aleksandra Baster, Zbigniew Rajfur, Zenon Mitchell, Judy A. Pyrc, Krzysztof Vet Res Research Article Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0551-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-07-03 2018 /pmc/articles/PMC6029178/ /pubmed/29970183 http://dx.doi.org/10.1186/s13567-018-0551-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Szczepanski, Artur
Owczarek, Katarzyna
Milewska, Aleksandra
Baster, Zbigniew
Rajfur, Zenon
Mitchell, Judy A.
Pyrc, Krzysztof
Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title_full Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title_fullStr Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title_full_unstemmed Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title_short Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
title_sort canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to hrt-18g cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029178/
https://www.ncbi.nlm.nih.gov/pubmed/29970183
http://dx.doi.org/10.1186/s13567-018-0551-9
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