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Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture
To reveal growth properties of Chlorella sorokiniana UTEX 1230, four monosaccharides (glucose, fructose, galactose and xylose) were individually supplemented into medium as carbon sources for the cultivation of C. sorokiniana UTEX 1230. Supplementation with glucose increased OD(750), biomass and lip...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029798/ https://www.ncbi.nlm.nih.gov/pubmed/29969497 http://dx.doi.org/10.1371/journal.pone.0199873 |
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author | Chai, Shuaijie Shi, Jianan Huang, Teng Guo, Yalu Wei, Jian Guo, Meicen Li, Liyun Dou, Shijuan Liu, Lijuan Liu, Guozhen |
author_facet | Chai, Shuaijie Shi, Jianan Huang, Teng Guo, Yalu Wei, Jian Guo, Meicen Li, Liyun Dou, Shijuan Liu, Lijuan Liu, Guozhen |
author_sort | Chai, Shuaijie |
collection | PubMed |
description | To reveal growth properties of Chlorella sorokiniana UTEX 1230, four monosaccharides (glucose, fructose, galactose and xylose) were individually supplemented into medium as carbon sources for the cultivation of C. sorokiniana UTEX 1230. Supplementation with glucose increased OD(750), biomass and lipid yield but decreased protein abundance per unit dry weight of biomass under all concentrations examined, the maximum OD(750), biomass and lipid yield increased 2.04, 6.78 and 12.43 times, respectively, compared with autotrophic controls. A low concentration of glucose (<4 g/L) simultaneously promoted the biosynthesis of chlorophylls and protein abundance per unit culture volume, but decreased the lipid content per unit dry weight of biomass and all supplemented glucose can be exhausted within 7 days. Higher glucose concentrations (≥4 g/L) decreased the biosynthesis of chlorophylls and protein abundance per unit culture volume, but increased the lipid content per unit dry weight of biomass. In glucose supplemented scenario, C. sorokiniana UTEX 1230 growth was light-independent. Supplementation with fructose promoted C. sorokiniana UTEX 1230 growth to a much lesser extent compared with glucose, whereas supplementation with galactose had no effect and supplementation with xylose even inhibited growth. Our findings represent basic experimental data on the effect of monosaccharides and can serve as the basis for a robust cultivation system to increase biomass and lipid yield. |
format | Online Article Text |
id | pubmed-6029798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60297982018-07-19 Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture Chai, Shuaijie Shi, Jianan Huang, Teng Guo, Yalu Wei, Jian Guo, Meicen Li, Liyun Dou, Shijuan Liu, Lijuan Liu, Guozhen PLoS One Research Article To reveal growth properties of Chlorella sorokiniana UTEX 1230, four monosaccharides (glucose, fructose, galactose and xylose) were individually supplemented into medium as carbon sources for the cultivation of C. sorokiniana UTEX 1230. Supplementation with glucose increased OD(750), biomass and lipid yield but decreased protein abundance per unit dry weight of biomass under all concentrations examined, the maximum OD(750), biomass and lipid yield increased 2.04, 6.78 and 12.43 times, respectively, compared with autotrophic controls. A low concentration of glucose (<4 g/L) simultaneously promoted the biosynthesis of chlorophylls and protein abundance per unit culture volume, but decreased the lipid content per unit dry weight of biomass and all supplemented glucose can be exhausted within 7 days. Higher glucose concentrations (≥4 g/L) decreased the biosynthesis of chlorophylls and protein abundance per unit culture volume, but increased the lipid content per unit dry weight of biomass. In glucose supplemented scenario, C. sorokiniana UTEX 1230 growth was light-independent. Supplementation with fructose promoted C. sorokiniana UTEX 1230 growth to a much lesser extent compared with glucose, whereas supplementation with galactose had no effect and supplementation with xylose even inhibited growth. Our findings represent basic experimental data on the effect of monosaccharides and can serve as the basis for a robust cultivation system to increase biomass and lipid yield. Public Library of Science 2018-07-03 /pmc/articles/PMC6029798/ /pubmed/29969497 http://dx.doi.org/10.1371/journal.pone.0199873 Text en © 2018 Chai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chai, Shuaijie Shi, Jianan Huang, Teng Guo, Yalu Wei, Jian Guo, Meicen Li, Liyun Dou, Shijuan Liu, Lijuan Liu, Guozhen Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title | Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title_full | Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title_fullStr | Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title_full_unstemmed | Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title_short | Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
title_sort | characterization of chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029798/ https://www.ncbi.nlm.nih.gov/pubmed/29969497 http://dx.doi.org/10.1371/journal.pone.0199873 |
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