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Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen

During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector pro...

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Autores principales: Dagdas, Yasin F, Pandey, Pooja, Tumtas, Yasin, Sanguankiattichai, Nattapong, Belhaj, Khaoula, Duggan, Cian, Leary, Alexandre Y, Segretin, Maria E, Contreras, Mauricio P, Savage, Zachary, Khandare, Virendrasinh S, Kamoun, Sophien, Bozkurt, Tolga O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029844/
https://www.ncbi.nlm.nih.gov/pubmed/29932422
http://dx.doi.org/10.7554/eLife.37476
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author Dagdas, Yasin F
Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y
Segretin, Maria E
Contreras, Mauricio P
Savage, Zachary
Khandare, Virendrasinh S
Kamoun, Sophien
Bozkurt, Tolga O
author_facet Dagdas, Yasin F
Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y
Segretin, Maria E
Contreras, Mauricio P
Savage, Zachary
Khandare, Virendrasinh S
Kamoun, Sophien
Bozkurt, Tolga O
author_sort Dagdas, Yasin F
collection PubMed
description During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles.
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spelling pubmed-60298442018-07-05 Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen Dagdas, Yasin F Pandey, Pooja Tumtas, Yasin Sanguankiattichai, Nattapong Belhaj, Khaoula Duggan, Cian Leary, Alexandre Y Segretin, Maria E Contreras, Mauricio P Savage, Zachary Khandare, Virendrasinh S Kamoun, Sophien Bozkurt, Tolga O eLife Cell Biology During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles. eLife Sciences Publications, Ltd 2018-06-22 /pmc/articles/PMC6029844/ /pubmed/29932422 http://dx.doi.org/10.7554/eLife.37476 Text en © 2018, Dagdas et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Dagdas, Yasin F
Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y
Segretin, Maria E
Contreras, Mauricio P
Savage, Zachary
Khandare, Virendrasinh S
Kamoun, Sophien
Bozkurt, Tolga O
Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title_full Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title_fullStr Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title_full_unstemmed Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title_short Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen
title_sort host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029844/
https://www.ncbi.nlm.nih.gov/pubmed/29932422
http://dx.doi.org/10.7554/eLife.37476
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